製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Nkx2.2
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Cow, Dog, Zebrafish
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Nkx2.2.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in Human placenta tissue lysate as well as the following whole cell lysates: U373; U87mg.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab79916 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 30 kDa).

ターゲット情報

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  • All lanes : Anti-Nkx2.2 antibody (ab79916) at 1 µg/ml

    Lane 1 : Human placenta tissue lysate - total protein (ab29745)
    Lane 2 : U373 MG (Human glioblastoma-astrocytoma, epithelial-like cell line) Nuclear Lysate (ab14902)
    Lane 3 : U-87 MG (Human glioblastoma astrocytoma) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 30 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 27 kDa,70 kDa,78 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes

プロトコール

参考文献

This product has been referenced in:
  • Xia CQ  et al. C-Abl inhibitor imatinib enhances insulin production by ß cells: c-Abl negatively regulates insulin production via interfering with the expression of NKx2.2 and GLUT-2. PLoS One 9:e97694 (2014). WB ; Mouse . Read more (PubMed: 24835010) »

See 1 Publication for this product

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