製品の概要
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製品名Anti-NKRF antibody [2639C1a]
NKRF 一次抗体 製品一覧 -
製品の詳細Mouse monoclonal [2639C1a] to NKRF
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由来種Mouse
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アプリケーション適用あり: WB, Dot blotmore details
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種交差性交差種: Human
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免疫原
Recombinant fragment of human NKRF
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ポジティブ･コントロール
- ウェスタン・ブロット用ポジティブコントロールRecombinant Human NKRF protein
- Recombinant human NKRF.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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バッファーPreservative: 0.05% Sodium Azide
Constituents: 1% BSA, PBS (8.0mM Sodium phosphate, 3.0mM Potassium chloride, 140mM Sodium chloride, 1.5mM Potassium phosphate, pH 7.4) -
Concentration information loading... -
精製度Protein G purified
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特記事項（精製）ab71963 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane.
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ポリ/モノモノクローナル
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クローン名2639C1a
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アイソタイプIgG2b
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研究分野
関連製品
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab71963 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | Use at an assay dependent dilution. Detects a band of approximately 40 kDa (predicted molecular weight: 78 kDa). | |
| Dot blot | Use at an assay dependent dilution. |
ターゲット情報
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関連性NKRF is a transcription factor that interacts with specific negative regulatory elements (NREs) to mediate transcriptional repression of certain NK-kappa-B-responsive genes. NKRF localizes predominantly to the nucleolus with a small fraction found in the nucleoplasm and cytoplasm. o involved in the regulation of IL-8 transcription.
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細胞内局在Nuclear
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参照データベース
- Entrez Gene: 55922 Human
- Omim: 300440 Human
- SwissProt: O15226 Human
- Unigene: 437084 Human
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別名
- ITBA4 antibody
- NF kappa B repressing factor antibody
- NFKB repressing factor antibody
see all
画像
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![Western blot - NKRF antibody [2639C1a] (ab71963)](http://a.abcam.com/ps/datasheet/Images/71/ab71963/ab71963.gif)
Western blot - NKRF antibody [2639C1a] (ab71963)Anti-NKRF antibody [2639C1a] (ab71963) + immunising recombinant protein
Predicted band size: 78 kDa
Observed band size: 40 kDa (why is the actual band size different from the predicted?)
The molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the protein was used.
プロトコール
参考文献
ab71963 has not yet been referenced specifically in any publications.
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"