The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 0.125-0.5µg for 105-8 cells. Perform staining a final volume of 100µl.
Receptor for MICA, MICB, ULBP1, ULBP2, ULBP3 (ULBP2>ULBP1>ULBP3) and ULBP4. Plays a role as a receptor for the recognition of MHC class I HLA-E molecules by NK cells and some cytotoxic T-cells. Involved in the immune surveillance exerted by T- and B-lymphocytes.
Natural killer cells. Expressed on essentially all CD56+CD3- NK cells from freshly isolated PBMC. Also detected in gamma-delta cells and CD8+ alpha-beta T-cells. Expressed in interferon-producing killer dendritic cells (IKDCs).
Staining of C57BL/6 splenocytes with anti-NK1.1 APC-conjugated and either 0.125µg of Rat IgG1k isotype control Phycoerythrin-conjugated (left) or 0.125µg of ab95798 (right). Total viable cells were used for analysis.