Anti-NKG2A 抗体 (ab96319)
Key features and details
- Rabbit polyclonal to NKG2A
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-NKG2A antibody
NKG2A 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to NKG2A -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, IHC-P, WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide within Human NKG2A (N terminal). The exact sequence is proprietary. Carrier protein conjugated.
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ポジティブ・コントロール
- WB: A431 and HepG2 cell lysate. ICC/IF: HeLa cells. IHC-P: H1299 xenograft.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab96319の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
1/100 - 1/1000.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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WB |
1/500 - 1/3000. Predicted molecular weight: 26 kDa.
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特記事項 |
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ICC/IF
1/100 - 1/1000. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
1/500 - 1/3000. Predicted molecular weight: 26 kDa. |
ターゲット情報
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機能
Plays a role as a receptor for the recognition of MHC class I HLA-E molecules by NK cells and some cytotoxic T-cells. -
組織特異性
Natural killer cells. -
配列類似性
Contains 1 C-type lectin domain. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 3821 Human
- Omim: 161555 Human
- SwissProt: P26715 Human
- Unigene: 512576 Human
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別名
- C lectin type II protein antibody
- CD159 antigen like family member A antibody
- CD159 antigen-like family member A antibody
see all
画像
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Paraffin-embedded H1299 xenograft stained for NKG2A using ab96319 at a 1/500 dilution in immunohistochemistry.
Antigen Retrieval: EDTA based, pH 8.0 buffer, 15 mins.
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All lanes : Anti-NKG2A antibody (ab96319) at 1/1000 dilution
Lane 1 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 26 kDa
12% SDS PAGE -
Methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeled for NKG2A using ab96319 at a 1/200 dilution in immunocytochemistry.
Bottom panel co-stained with Hoechst 33342.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab96319 は論文での使用が確認できていません。