Rabbit polyclonal to NKF3 kinase family member
antigen sequence: EPNYESPSSN NQDKDSSQAS KSSIKVPETH KAVLALRLEE KDGKIAVQTE KEESKASTDV AGQAVTINLV PTEEQAKPYR VVNLEQPLCK, corresponding to internal sequence amino acids 384-473 of Human NKF3 kinase family member.
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Preservative: 0.02% Sodium azide Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Tyrosine kinase that may play a role in cell spreading and migration on fibronectin. May directly or indirectly affect phosphorylation levels of cytoskeleton-associated proteins MAPK1/ERK and PXN.
Belongs to the protein kinase superfamily.
Contains 1 protein kinase domain.
Phosphorylated on tyrosine in a CSK-dependent manner in response to adhesion to fibronectin and to EGF stimulation. Autophosphorylated in vitro.
Cytoplasm > cytoskeleton. Cell junction > focal adhesion. Colocalizes with F-actin in serum-rich medium. Actin colocalization is reduced during serum starvation.
Information by UniProt
Immunocytochemistry/ Immunofluorescence - Anti-NKF3 kinase family member antibody (ab121869)
Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleus but not nucleoli, plasma membrane and cytoplasm. Recommended concentration of ab121869 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NKF3 kinase family member antibody (ab121869)
ab121869, at 1/1500 dilution, staining NKF3 kinase family member in glandular cells of paraffin-embedded Human stomach tissue by Immunohistochemistry.
has not yet been referenced specifically in any publications.
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