製品の概要

  • 製品名
  • 製品の詳細
    Mouse polyclonal to NKD2
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    NKD2 full length human protein

  • ポジティブ・コントロール
    • NKD2 transfected 293T cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab67911 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 37 kDa (predicted molecular weight: 50 kDa).

ターゲット情報

  • 機能
    Cell autonomous antagonist of the canonical Wnt signaling pathway. May activate a second Wnt signaling pathway that controls planar cell polarity (By similarity). Required for processing of TGFA and for targeting of TGFA to the basolateral membrane of polarized epithelial cells.
  • 組織特異性
    Expressed in kidney, lung, pancreas and spleen.
  • 配列類似性
    Belongs to the NKD family.
    Contains 1 EF-hand domain.
  • 発生段階
    Expressed in fetal kidney and lung.
  • ドメイン
    The N-terminal domain comprising the first 217 amino acid residues is mostly unstructured.
  • 翻訳後修飾
    Ubiquitinated, leading to rapid proteasomal degradation. Interaction with TGFA interferes with RNF25 binding and protects against ubiquitination mediated by RNF25.
  • 細胞内局在
    Cell membrane. Cytoplasm. Cytoplasmic vesicle.
  • Information by UniProt
  • 参照データベース
  • 別名
    • hNkd2 antibody
    • Naked 2 antibody
    • Naked-2 antibody
    • nkd2 antibody
    • NKD2_HUMAN antibody
    • Protein naked cuticle homolog 2 antibody
    see all

画像

  • All lanes : Anti-NKD2 antibody (ab67911) at 1/500 dilution

    Lane 1 : NKD2 transfected 293T cell lysate
    Lane 2 : non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 50 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)

プロトコール

参考文献

ab67911 has not yet been referenced specifically in any publications.

レビューと Q&A

Thank you for contacting Abcam. The antibody ab67911 has not been tested in IHC and so I cannot provide any protocol information for you. As it has not been tested in IHC, I can offer you our testing discount. The details of which are below. If there i...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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