Anti-NKCC1 抗体 (ab99558)
Key features and details
- Goat polyclonal to NKCC1
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-NKCC1 antibody
NKCC1 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to NKCC1 -
由来種
Goat -
アプリケーション
適用あり: IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Cow, Dog, Cynomolgus monkey -
免疫原
Synthetic peptide corresponding to Human NKCC1 aa 750-850 (internal sequence). NP_001037.1
Database link: P55011 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading...
-
精製度
Immunogen affinity purified -
特記事項(精製)
Ammonium sulphate precipitation followed by antigen affinity chromatography. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab99558の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IHC-P | (1) |
Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
特記事項 |
---|
IHC-P
Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
-
機能
Electrically silent transporter system. Mediates sodium and chloride reabsorption. Plays a vital role in the regulation of ionic balance and cell volume. -
組織特異性
Expressed in many tissues. -
配列類似性
Belongs to the SLC12A transporter family. -
細胞内局在
Membrane. - Information by UniProt
-
参照データベース
- Entrez Gene: 286845 Cow
- Entrez Gene: 481490 Dog
- Entrez Gene: 6558 Human
- Entrez Gene: 20496 Mouse
- Entrez Gene: 83629 Rat
- Omim: 600840 Human
- SwissProt: P55011 Human
- SwissProt: P55012 Mouse
see all -
別名
- Basolateral Na-K-Cl symporter antibody
- BSC antibody
- BSC2 antibody
see all
データシートおよび資料
-
Datasheet download
参考文献 (4)
ab99558 は 4 報の論文で使用されています。
- He D et al. Asthmatic Airway Vagal Hypertonia Involves Chloride Dyshomeostasis of Preganglionic Neurons in Rats. Front Neurosci 14:31 (2020). PubMed: 32082109
- McNeill JK et al. The Excitatory Effects of GABA within the Suprachiasmatic Nucleus: Regulation of Na-K-2Cl Cotransporters (NKCCs) by Environmental Lighting Conditions. J Biol Rhythms 35:275-286 (2020). PubMed: 32406304
- Ma H et al. NKCC1 promotes EMT-like process in GBM via RhoA and Rac1 signaling pathways. J Cell Physiol 234:1630-1642 (2019). PubMed: 30159893
- Rohr KE et al. Seasonal plasticity in GABAA signaling is necessary for restoring phase synchrony in the master circadian clock network. Elife 8:N/A (2019). PubMed: 31746738