製品の概要
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製品名Anti-NKCC1 antibody
NKCC1 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to NKCC1
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由来種Rabbit
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特異性This antibody reacts specifically with human 132 kDa NKCC1 protein.
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アプリケーション適用あり: IHC-FoFr, WB, IHC-Pmore details
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種交差性交差種: Mouse, Rat, Human
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免疫原
Synthetic peptide derived from the N terminal region of human NKCC1.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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バッファーPreservative: None
Constituents: Whole serum -
精製度Whole antiserum
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
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Immunohistochemistry kits
アプリケーション
Our Abpromise guarantee covers the use of ab59791 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-FoFr | Use at an assay dependent concentration. PubMed: 21461058 | |
| WB | 1/100 - 1/1000. Predicted molecular weight: 130 kDa. | |
| IHC-P | Use a concentration of 5 µg/ml. |
ターゲット情報
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機能Electrically silent transporter system. Mediates sodium and chloride reabsorption. Plays a vital role in the regulation of ionic balance and cell volume.
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組織特異性Expressed in many tissues.
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配列類似性Belongs to the SLC12A transporter family.
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細胞内局在Membrane.
- Information by UniProt
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参照データベース
- Entrez Gene: 6558 Human
- Entrez Gene: 20496 Mouse
- Entrez Gene: 83629 Rat
- Omim: 600840 Human
- SwissProt: P55011 Human
- SwissProt: P55012 Mouse
- Unigene: 162585 Human
- Unigene: 712970 Human
see all -
別名
- Basolateral Na-K-Cl symporter antibody
- BSC antibody
- BSC2 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NKCC1 antibody (ab59791)This image is courtesy of an Abreview submitted by Carl Hobbsab59791 staining NKCC1 in Mouse salivary gland tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 1% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/600 in blocking buffer) for 2 hours at 21°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/250) was used as the secondary antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NKCC1 antibody (ab59791)This image is courtesy of an anonymous Abreviewab59791 staining NKCC1 in Rat brain stem tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.1% Trition and blocked with 5% BSA for 3 hours at 20°C; antigen retrieval was by heat mediation in a citrate sodium buffer. Samples were incubated with primary antibody (1/1000) for 15 hours at 4°C. An Alexa Fluor® 568-conjugated Goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NKCC1 antibody (ab59791)IHC image of ab59791 staining in Human breast cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab59791, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
参考文献
This product has been referenced in:
- Yan X et al. Pretreatment with AQP4 and NKCC1 Inhibitors Concurrently Attenuated Spinal Cord Edema and Tissue Damage after Spinal Cord Injury in Rats. Front Physiol 9:6 (2018). Read more (PubMed: 29403391) »
- Shih HC et al. Targeting brain-derived neurotrophic factor in the medial thalamus for the treatment of central poststroke pain in a rodent model. Pain 158:1302-1313 (2017). WB ; Rat . Read more (PubMed: 28394853) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"