Anti-Nitrotyrosine 抗体 [HM11] (ab94883)


  • 製品名Anti-Nitrotyrosine antibody [HM11]
    Nitrotyrosine 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [HM11] to Nitrotyrosine
  • 特異性ab94883 reacts with the free amino acid nitrotyrosine, as well as with proteins containing nitrotyrosine.
  • アプリケーション適用あり: ELISA, IHC-P, IHC-Fr, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    The molecule nitrotyrosine

  • ポジティブ・コントロール
    • Western Blot: Mouse kidney lysate, mouse optic nerve, retina, spinal cord and brain lysates, rat aorta lysate Paraffin sections: Human lung tissue



Our Abpromise guarantee covers the use of ab94883 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.
IHC-P 1/10.
IHC-Fr 1/10.
WB 1/10.


  • 関連性The cellular production of highly reactive nitrogen species derived from nitric oxide, such as peroxynitrite, nitrogen dioxide and nitryl chloride, leads to the nitration of tyrosine resides in tissue proteins. The extent of protein nitrotyrosine formation provides an index of the production of reactive nitrogen species and potential cell damage over a period of time. Nitrotyrosine can be measured by amino-acid analysis of protein hydrolysates and detected, estimated semi-quantitatively and located in cells and tissues by immunocytochemical techniques using antibodies directed against the nitrotyrosine hapten.

Anti-Nitrotyrosine antibody [HM11] (ab94883) 使用論文

ab94883 has not yet been referenced specifically in any publications.

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Thank you for providing that information.

Having looked over the protocol you provided, I do not think that there is any advice I could give that would help resolve the issues that you are having. Therefore I would be happy o either replace or...

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Thank you for contacting Abcam.

I am sorry about the problems you have been having with ab7048, which is still in the catalogue and can be found at the link below:
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Thank you for your enquiry. I am sorry to confirm that non of our Oxoguanine 8, Nitrotyrosine, and Malondialdehyde antibodies have been tested with half Karnovsky's fixative. Regrettably, this means we therefore cannot confirm and guarantee how well...

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Thanks for your reply. I believe that these are the controls the testing lab uses in their QC process, but I will contact them to confirm that and also ask for additional references. For controls in IHC, you can use a slide of the same tissue ty...

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Thank you for contacting us. We recommend using the following controls for ab94883: Positive Controls: Western Blot: mouse kidney lysate (ab29305), mouse optic nerve, retina , spinal cord and brain lysates, rat aorta lysate Paraffin secti...

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