製品の概要

  • 製品名
    Anti-Nitro tyrosine antibody [HM.11]
    Nitro tyrosine 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [HM.11] to Nitro tyrosine
  • 特異性
    This antibody reacts with nitrotyrosine, both with the free amino acid as well as with proteins containing nitrotyrosine. The antibody does not recognize other antigens with a nitrotyrosine-like structure such as tyrosine, 3-chlorotyrosine, phosphotyrosine and phenylalanine.
  • アプリケーション
    適用あり: WB, ELISA, IHC-P, IHC-Frmore details
  • 免疫原

    Synthetic peptide corresponding to Nitro tyrosine (nitrated ) conjugated to Keyhole Limpet Haemocyanin (KLH).

  • ポジティブ・コントロール
    • WB: mouse kidney lysate, mouse optic nerve, retina , spinal cord and brain lysates, rat aorta lysate(see Abreviews) IHC-P: human lung tissue (see Abreview)

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 0.1% BSA, PBS
    WARNING!!: Do not add sodium azide or nitric containing perservative as it will damage the antibody.
  • Concentration information loading...
  • 精製度
    Protein G purified
  • ポリ/モノ
    モノクローナル
  • クローン名
    HM.11
  • アイソタイプ
    IgG2b
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab7048 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
IHC-P 1/200 - 1/400. 10% formalin fixation; 3% H2O2 to block endogenous peroxidases; Citrate buffer pH 6.0 for 1 min at 100°C as antigen retrieval treatment
IHC-Fr Use at an assay dependent concentration. Acetone fixation 10 min -20°C; block endogenous peroxidase by 0.3 % H2O2 in PBS (or methanol for intracellular staining); block with 10% NGS or 5 % BSA for 30 min.

ターゲット情報

  • 関連性
    Nitric oxide (NO) is a product of the enzymatic conversion of arginine to citrulline by nitric oxide synthase. NO reacts rapidly with superoxide to form peroxynitrite. At physiological pH and in the presence of transition metals, peroxynitrite undergoes heterolytic cleavage to form hydroxyl anion and nitronium ion, the latter of which nitrates protein tyrosine residues. Thus, the presence of nitrotyrosine on proteins can be used as a marker for peroxynitrite formation in vivo. The presence of nitrotyrosine has been detected in various inflammatory processes including atherosclerotic placques.
  • 別名
    • NO tyrosine antibody
    • nTyr antibody

画像

  • Immunofluorescence analysis of 22Rv1 cells (Human prostate carcinoma epithelial cell line) treated with JS-K, staining Nitro tyrosine (green) with ab7048.

    Cells were fixed with paraformaldehyde, permeabilized in 0.2% Triton X-100 and blocked with 1% BSA. Cells were incubated with primary antibody (1/25) for 30 min at 37°C. An AlexaFluor488®-conjugated anti-mouse IgG (1/100) was used as the secondary antibody.
  • ab7048 at 2 µg/ml staining Nitrotyrosine in Human liver from a severely obese patient. ab7048 was incubated for 1 hour at room temperature.

参考文献

This product has been referenced in:
  • Cowley AW  et al. Evidence of the Importance of Nox4 in Production of Hypertension in Dahl Salt-Sensitive Rats. Hypertension 67:440-50 (2016). Read more (PubMed: 26644237) »
  • Rice KM  et al. Differential regulation of apoptosis in slow and fast twitch muscles of aged female F344BN rats. Age (Dordr) 37:30 (2015). Read more (PubMed: 25813803) »

See all 29 Publications for this product

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rhesus monkey Tissue sections (Colon)
Specification
Colon
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer
Permeabilization
Yes - 10 mM Tris-HCl pH-8.0, 150 mM NaCl containing 0.1% Triton-X-100
Blocking step
3%BSA and 10% normal goat serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

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Verified customer

投稿 Apr 11 2013

Vielen Dank für Ihre Anfrage und dafür, dass Sie sich die Zeit genommen haben, unseren Fragebogen auszufüllen.

Es tut mir leid, dass Sie Probleme mit unserem Antikörper hatten.

Um Ihr Problem zu lösen, mö...

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There is a western blot on our online datasheet for ab131379 http://www.abcam.com/bovine-serum-albumin-protein-nitrated-ab131379.html illustrating this protein to be detectable by ab110282, another Anti-Nitrotyrosine antibody. So we will guar...

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Es freut mich zu hören, dass Sie unser Angebot unseren Antikörper ab7048 in IP zu testen, annehmen möchten. Wie am Telefon besprochen, können Sie den Gutscheincode zum Kauf eines weiteren Antikörpers einsetzen, sobal...

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Vielen Dank für Ihren Anruf und für Ihr Interesse an unseren Produkten.

Unseres Wissens nach wurde Anti-Nitro tyrosine antibody [HM.11] (ab7048) bisher noch nicht in IP getestet. Falls Sie dies selbst testen möchten, kann ich Ih...

Read More

Our nitrated BSA (ab131379) should be a good control for use with ab7048.

Thank you for calling Abcam earlier today.

I am sorry about the issues you have been having with ab7048. Asrequested I am sending you a new vial of the antibody, ab78163and you should receive that tomorrow morning. For your reference, your new...

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Thank you for providing that information.

Having looked over the protocol you provided, I do not think that there is any advice I could give that would help resolve the issues that you are having. Therefore I would be happy o either replace or...

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Thank you for contacting Abcam.

I am sorry about the problems you have been having with ab7048, which is still in the catalogue and can be found at the link below:

http://www.abcam.com/Nitro-tyrosine-antibody-HM-11-ab7048.html
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Thank you for calling Abcam earlier today.

As we discussed, I am sending you a new vial of antibody, ab42789 as a free of charge replacement. Your new order number is *********.

The antibody is covered under our Abpromise to work in...

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1-10 of 34 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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