The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: range: 1/5000 to 1/40000.
WB: range: 1/5000 to 1/40000.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Nitric oxide (NO) is a product of the enzymatic conversion of arginine to citrulline by nitric oxide synthase. NO reacts rapidly with superoxide to form peroxynitrite. At physiological pH and in the presence of transition metals, peroxynitrite undergoes heterolytic cleavage to form hydroxyl anion and nitronium ion, the latter of which nitrates protein tyrosine residues. Thus, the presence of nitrotyrosine on proteins can be used as a marker for peroxynitrite formation in vivo. The presence of nitrotyrosine has been detected in various inflammatory processes including atherosclerotic placques.