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Our Abpromise guarantee covers the use of ab28673 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 µg/ml. Detects a band of approximately 90 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|IHC-P||Use at an assay dependent concentration.|
This image is courtesy of an anonymous Abreview
Western blot analysis using ab28673 at 1/2000.
Lane 1) Whole cell HeLa lysate
Lane 2) Recombinant purified UHRF2 protein
Lane 3) Recombinant purified UHRF1 protein
Lane 4) Whole cell HeLa lysate with UHRF1 knockdown (short hairpin RNA)
A HRP-conjugated donkey anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.