NIR Mitochondrial Membrane Potential Assay Kit (Microplate) (ab112150)
Key features and details
- Assay type: Direct
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Adherent cells, Suspension cells
製品の概要
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製品名
NIR Mitochondrial Membrane Potential Assay Kit (Microplate)
Mitochondrial Membrane Potential キット 製品一覧 -
検出方法
Fluorescent -
サンプルの種類
Adherent cells, Suspension cells -
アッセイタイプ
Direct -
製品の概要
ab112150 is designed to detect cell apoptosis by measuring the loss of the mitochondrial membrane potential. The collapse of mitochondrial membrane potential coincides with the opening of the mitochondrial permeability transition pores, leading to the release of cytochrome C into the cytosol, which in turn triggers other downstream events in the apoptotic cascade.
ab112150 NIR Mitochondria Membrane Potential Assay Kit provides all the essential components with an optimized assay method. This fluorometric assay uses our proprietary cationic MitoNIR Dye for the detection of the mitochondrial membrane potential change in cells. In normal cells, the red fluorescence intensity is increased when NIR Dye is accumulated in the mitochondria. However, in apoptotic cells, NIR stain intensity is decreased following the collapse of MMP. Cells stained with NIR Dye can be monitored fluorimetrically at Em = 660-680 nm with excitation at Ex = 620-640 nm.
ab112150 can be used for screening apoptosis activators and inhibitors. The assay can be performed in a convenient 96-well and 384-well fluorescence microtiter-plate format.
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特記事項
Related assays
Review the cell health assay guide to learn about kits to perform a cell viability assay, cytotoxicity assay and cell proliferation assay.
Review the metabolism assay guide to learn about assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also about how to assay metabolic function in live cells using your plate reader.
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試験プラットフォーム
Microplate reader
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 5 x 96 tests Assay Buffer A 1 x 50ml Assay Buffer B 1 x 25ml MitoNIR Dye 1 x 250µl -
研究分野
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関連性
Mitochondrial Membrane Potential is an important parameter of mitochondrial function used as an indicator of cell death. The collapse of the mitochondrial Membrane potential coincides with the opening of the mitochondrial permeability transition pores, leading to the release of cytochrome c into the cytosol, which in turn triggers other downstream events in the apoptotic cascade. -
別名
- mitochondrial membrane potential
関連製品
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Related Products
画像
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The decrease in NIR fluorescence with the addition of FCCP in HeLa cells.
HeLa cells were dye loaded with NIR alone, or in the presence of 20 µM FCCP for 15 minutes. The fluorescence intensity of NIR was measured 30 minutes after adding Assay buffer B (Component C) with a microplate reader at Ex/Em = 640/680 nm (cut off 665 nm).
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab112150 は論文での使用が確認できていません。