Anti-NIPP1 抗体 (ab5300)
Key features and details
- Goat polyclonal to NIPP1
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-NIPP1 antibody
NIPP1 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to NIPP1 -
由来種
Goat -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Cow, Dog -
免疫原
Synthetic peptide corresponding to Human NIPP1 aa 114-127 (C terminal).
Sequence:C-EAWPGKKPTPSLLI
(Peptide available asab23096) -
ポジティブ・コントロール
- WB: HeLa, HepG2, Jurkat and NIH3T3 nuclear lysates. IHC-P: Human thymus tissue.
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特記事項
This gene, through alternative splicing, encodes three different isoforms. Two of the protein isoforms encoded by this gene are specific inhibitors of type 1 serine/threonine protein phosphatases and can bind but not cleave RNA. The third protein isoform lacks the phosphatase inhibitory function but is a single-strand endoribonuclease comparable to RNase E of E. coli. This isoform requires magnesium for its function and cleaves specific sites in A+U-rich regions of RNA.
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製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris buffered saline, 0.5% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
一次抗体 備考
This gene, through alternative splicing, encodes three different isoforms. Two of the protein isoforms encoded by this gene are specific inhibitors of type 1 serine/threonine protein phosphatases and can bind but not cleave RNA. The third protein isoform lacks the phosphatase inhibitory function but is a single-strand endoribonuclease comparable to RNase E of E. coli. This isoform requires magnesium for its function and cleaves specific sites in A+U-rich regions of RNA. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab5300の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
Use a concentration of 2.5 - 3.8 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).
1 hour primary incubation is recommended for this product. |
特記事項 |
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IHC-P
Use a concentration of 2.5 - 3.8 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa). 1 hour primary incubation is recommended for this product. |
ターゲット情報
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機能
Inhibitor subunit of the major nuclear protein phosphatase-1 (PP-1). It has RNA-binding activity but does not cleave RNA and may target PP-1 to RNA-associated substrates. May also be involved in pre-mRNA splicing. Binds DNA and might act as a transcriptional repressor. Seems to be required for cell proliferation.
Isoform Gamma is a site-specific single-strand endoribonuclease that cleaves single strand RNA 3' to purines and pyrimidines in A+U-rich regions. It generates 5'-phosphate termini at the site of cleavage. This isoform does not inhibit PP-1. May be implicated in mRNA splicing. -
組織特異性
Ubiquitously expressed, with highest levels in heart and skeletal muscle, followed by brain, placenta, lung, liver and pancreas. Less abundant in kidney. The concentration and ratio between isoforms is cell-type dependent. Isoform Alpha (>90%) and isoform Beta were found in brain, heart and kidney. Isoform Gamma is mainly found in B-cells and T-lymphocytes, and has been found in 293 embryonic kidney cells. -
配列類似性
Contains 1 FHA domain. -
ドメイン
Has a basic N- and C-terminal and an acidic central domain. -
翻訳後修飾
May be inactivated by phosphorylation on Ser-199 or Ser-204 (By similarity). Phosphorylated by Lyn in vitro on Tyr-264, and also on Tyr-335 in the presence of RNA. -
細胞内局在
Nucleus. Nucleus speckle. Primarily, but not exclusively, nuclear and Cytoplasm. Found mainly in the cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 282319 Cow
- Entrez Gene: 5511 Human
- Entrez Gene: 100336 Mouse
- Entrez Gene: 313030 Rat
- Omim: 602636 Human
- SwissProt: Q28147 Cow
- SwissProt: Q12972 Human
- SwissProt: Q8R3G1 Mouse
see all -
別名
- Activator of RNA decay antibody
- ARD 1 antibody
- ARD-1 antibody
see all
画像
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All lanes : Anti-NIPP1 antibody (ab5300) at 1 µg/ml
Lane 1 : HeLa nuclear lysate in RIPA buffer
Lane 2 : HepG2 nuclear lysate in RIPA buffer
Lane 3 : Jurkat nuclear lysate in RIPA buffer
Lane 4 : NIH3T3 nuclear lysate in RIPA buffer
Lysates/proteins at 35 µg per lane.
Predicted band size: 40 kDaPrimary incubation for 1 hour. Detected using chemiluminescence.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (1)
ab5300 は 1 報の論文で使用されています。
- Nuytten M et al. The transcriptional repressor NIPP1 is an essential player in EZH2-mediated gene silencing. Oncogene 27:1449-60 (2008). WB . PubMed: 17724462