特記事項Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA).
To ensure stability, we recommend aliquoting the extracts into single-use fractions and then storing them at -80ºC.
保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
背景The cell line NIH:OVCAR-3 has been established from the malignant ascites of a patient with progressive adenocarcinoma of the ovary after combination chemotherapy with cyclophosphamide, Adriamycin, and cisplatin. OVCAR-3 grows as a cobblestone-like monolayer with foci of multilayering, is tumorigenic in athymic mice, clones in agarose, and has an abnormal karyotype which includes a homogeneous staining region and a double minute chromosome. The cultured cells have an epithelial morphology and contain cytoplasmic androgen- and estrogen-binding macromolecules with the specificity of the respective steroid hormone receptors. OVCAR-3 may be of use for investigations as to the significance of androgens and estrogens and the mechanisms of cytotoxic drug resistance in ovarian cancer.
NIH OVCAR-3 nuclear extract lysate (ab14879) 使用論文
has not yet been referenced specifically in any publications.