製品の概要

  • 製品名Anti-NIFK (phospho T234) antibody
    NIFK 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to NIFK (phospho T234)
  • 特異性ab61110 detects endogenous levels of NIFK only when phosphorylated at threonine 234.
  • アプリケーション適用あり: ELISA, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse
  • 免疫原

    Synthetic peptide derived from human NIFK around the phosphorylation site of threonine 234 (G-P-TP-P-V).

  • ポジティブ・コントロール
    • Human brain tissue and HuvEC cells.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab61110 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/40000.
IHC-P 1/50 - 1/100.
ICC/IF 1/100 - 1/500.

ターゲット情報

  • 配列類似性Contains 1 RRM (RNA recognition motif) domain.
  • 翻訳後修飾Sequentially phosphorylated on Thr-238, Thr-234 and Ser-230. Thr-234 is phosphorylated only when Thr-238 is phosphorylated. Likewise, phosphorylation at Ser-230 requires that Thr-234 and Thr-238 are phosphorylated. Phosphorylation enhances MKI67 binding.
  • 細胞内局在Nucleus > nucleolus. Chromosome. Localizes to mitotic chromosomes in conjunction with MKI67.
  • Information by UniProt
  • 参照データベース
  • 別名
    • hNIFK antibody
    • MK67I_HUMAN antibody
    • MKI67 FHA Domain Interacting Nucleolar Phosphoprotein antibody
    • MKI67 FHA domain-interacting nucleolar phosphoprotein antibody
    • mki67ip antibody
    • NOPP34 antibody
    • Nucleolar phosphoprotein Nopp34 antibody
    • Nucleolar protein interacting with the FHA domain of pKI 67 antibody
    • Nucleolar protein interacting with the FHA domain of pKI-67 antibody
    • pKi67 antibody
    see all

Anti-NIFK (phospho T234) antibody 画像

  • ab61110 at a 1/50-1/100 dilution, staining human NIFK in brain tissue with (+) or without (-) immunising phospopeptide, using Immunohistochemistry, Paraffin embedded tissue
  • ab61110, at a 1/100-1/500 dilution, staining human NIFK in HuvEC cells with (+) or without (-) immunising phospopeptide, using Immunofluorescence

Anti-NIFK (phospho T234) antibody (ab61110) 使用論文

This product has been referenced in:
  • Booth DG  et al. Ki-67 is a PP1-interacting protein that organises the mitotic chromosome periphery. Elife 3:e01641 (2014). ICC/IF ; Human . Read more (PubMed: 24867636) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab61110.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"