製品の概要

  • 製品名Anti-NIFK antibody
    NIFK 一次抗体 製品一覧
  • 製品の詳細
    Mouse polyclonal to NIFK
  • アプリケーション適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length human NIFK, amino acids 1-293 (NP_115766.2)

  • ポジティブ・コントロール
    • Human kidney tissue lystae, NIFK transfected 293T cell lysate, and HeLa cells.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab89642 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
ICC/IF Use a concentration of 10 µg/ml.

ターゲット情報

  • 配列類似性Contains 1 RRM (RNA recognition motif) domain.
  • 翻訳後修飾Sequentially phosphorylated on Thr-238, Thr-234 and Ser-230. Thr-234 is phosphorylated only when Thr-238 is phosphorylated. Likewise, phosphorylation at Ser-230 requires that Thr-234 and Thr-238 are phosphorylated. Phosphorylation enhances MKI67 binding.
  • 細胞内局在Nucleus > nucleolus. Chromosome. Localizes to mitotic chromosomes in conjunction with MKI67.
  • Information by UniProt
  • 参照データベース
  • 別名
    • hNIFK antibody
    • MK67I_HUMAN antibody
    • MKI67 FHA Domain Interacting Nucleolar Phosphoprotein antibody
    • MKI67 FHA domain-interacting nucleolar phosphoprotein antibody
    • mki67ip antibody
    • NOPP34 antibody
    • Nucleolar phosphoprotein Nopp34 antibody
    • Nucleolar protein interacting with the FHA domain of pKI 67 antibody
    • Nucleolar protein interacting with the FHA domain of pKI-67 antibody
    • pKi67 antibody
    see all

Anti-NIFK antibody 画像

  • Anti-NIFK antibody (ab89642) at 1 µg/ml + human kidney tissue lysate at 50 µg

    Predicted band size : 34 kDa
    Observed band size : 34 kDa
  • All lanes : Anti-NIFK antibody (ab89642) at 1 µg/ml

    Lane 1 : NIFK transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 34 kDa
    Observed band size : 34 kDa
  • ab89642 at 10µg/ml staining NIFK in HeLa cells by Immunofluorescence.

Anti-NIFK antibody (ab89642) 使用論文

ab89642 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"