製品の概要

  • 製品名Anti-NIFK antibody
    NIFK 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to NIFK
  • 特異性ab61203 detects endogenous levels of total NIFK protein.
  • アプリケーション適用あり: WB, ELISA, ICC/IF, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse
  • 免疫原

    Synthetic non-phosphopeptide derived from human NIFK around the phosphorylation site of threonine 234.

  • ポジティブ・コントロール
    • Human brain tissue and HuvEc cells.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)ab61203 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab61203 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 34 kDa).
ELISA 1/5000.
ICC/IF 1/100 - 1/500.
IHC-P 1/50 - 1/100.

ターゲット情報

  • 配列類似性Contains 1 RRM (RNA recognition motif) domain.
  • 翻訳後修飾Sequentially phosphorylated on Thr-238, Thr-234 and Ser-230. Thr-234 is phosphorylated only when Thr-238 is phosphorylated. Likewise, phosphorylation at Ser-230 requires that Thr-234 and Thr-238 are phosphorylated. Phosphorylation enhances MKI67 binding.
  • 細胞内局在Nucleus > nucleolus. Chromosome. Localizes to mitotic chromosomes in conjunction with MKI67.
  • Information by UniProt
  • 参照データベース
  • 別名
    • hNIFK antibody
    • MK67I_HUMAN antibody
    • MKI67 FHA Domain Interacting Nucleolar Phosphoprotein antibody
    • MKI67 FHA domain-interacting nucleolar phosphoprotein antibody
    • mki67ip antibody
    • NOPP34 antibody
    • Nucleolar phosphoprotein Nopp34 antibody
    • Nucleolar protein interacting with the FHA domain of pKI 67 antibody
    • Nucleolar protein interacting with the FHA domain of pKI-67 antibody
    • pKi67 antibody
    see all

Anti-NIFK antibody 画像

  • ab61203 at 1/50 dilution staining NIFK in human brain by Immunohistochemistry, Paraffin-embedded tissue, in the absence (left) or presence (right) of the immunising peptide.
  • ab61203 at 1/100 dilution staining NIFK in HuvEc cells by Immunofluorescence, in the absence (left) or presence (right) of the immunising peptide.
  • All lanes : Anti-NIFK antibody (ab61203) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate (ab76999) with SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
    Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/3000 dilution

    Predicted band size : 34 kDa
    Observed band size : 41 kDa (why is the actual band size different from the predicted?)

Anti-NIFK antibody (ab61203) 使用論文

ab61203 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab61203.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"