Anti-NIFK 抗体 [18E148] (ab13880)

製品の概要

  • 製品名Anti-NIFK antibody [18E148]
    NIFK 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [18E148] to NIFK
  • アプリケーション適用あり: WB, Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide, corresponding to amino acids 100-200 of Human NIFK.

  • ポジティブ・コントロール
    • Ramos or HeLa whole cell lysate.
  • 特記事項Stable for 6 months at 4 °C.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.05% Sodium Azide
    Constituents: 0.2% Gelatin, PBS
  • Concentration information loading...
  • 精製度Protein G purified
  • ポリ/モノモノクローナル
  • クローン名18E148
  • アイソタイプIgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab13880 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.5 - 3 µg/ml. Predicted molecular weight: 34 kDa.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 配列類似性Contains 1 RRM (RNA recognition motif) domain.
  • 翻訳後修飾Sequentially phosphorylated on Thr-238, Thr-234 and Ser-230. Thr-234 is phosphorylated only when Thr-238 is phosphorylated. Likewise, phosphorylation at Ser-230 requires that Thr-234 and Thr-238 are phosphorylated. Phosphorylation enhances MKI67 binding.
  • 細胞内局在Nucleus > nucleolus. Chromosome. Localizes to mitotic chromosomes in conjunction with MKI67.
  • Information by UniProt
  • 参照データベース
  • 別名
    • hNIFK antibody
    • MK67I_HUMAN antibody
    • MKI67 FHA Domain Interacting Nucleolar Phosphoprotein antibody
    • MKI67 FHA domain-interacting nucleolar phosphoprotein antibody
    • mki67ip antibody
    • NOPP34 antibody
    • Nucleolar phosphoprotein Nopp34 antibody
    • Nucleolar protein interacting with the FHA domain of pKI 67 antibody
    • Nucleolar protein interacting with the FHA domain of pKI-67 antibody
    • pKi67 antibody
    see all

Anti-NIFK antibody [18E148] 画像

  • Overlay histogram showing Ramos cells stained with ab13880 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab13880, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Ramos cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-NIFK antibody [18E148] (ab13880) 使用論文

ab13880 has not yet been referenced specifically in any publications.

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