The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 145 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
NIF1 enhances transcriptional activation by ligand-bound nuclear hormone receptors. However, it does this not by direct interaction with the receptor, but by direct interaction with the nuclear hormone receptor transcriptional coactivator NRC. This protein may function by altering local chromatin structure.