Customer reviews and FAQs

製品の概要

  • 製品名Anti-Nidogen 2 antibody
    Nidogen 2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Nidogen 2
  • アプリケーション適用あり: WB, ELISA, IHC-Fr, Flow Cyt, ICC/IFmore details
  • 種交差性
    交差種: Mouse
  • 免疫原

    Recombinant full length protein (Mouse).

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab14513 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent concentration. Predicted molecular weight: 153 kDa.
ELISA Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. PubMed: 23211522ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/200. PubMed: 23409002

ターゲット情報

  • 機能Cell adhesion glycoprotein which is widely distributed in basement membranes. Binds to collagens I and IV, to perlecan and to laminin 1. Does not bind fibulins. It probably has a role in cell-extracellular matrix interactions.
  • 組織特異性Heart, placenta and bone. Less in pancreas, kidney and skeletal muscle.
  • 配列類似性Contains 5 EGF-like domains.
    Contains 5 LDL-receptor class B repeats.
    Contains 1 NIDO domain.
    Contains 1 nidogen G2 beta-barrel domain.
    Contains 2 thyroglobulin type-1 domains.
  • 翻訳後修飾Highly N- and O-glycosylated.
  • 細胞内局在Secreted > extracellular space > extracellular matrix > basement membrane.
  • Information by UniProt
  • 参照データベース
    • 別名
      • NID 2 antibody
      • NID-2 antibody
      • Nid2 antibody
      • NID2_HUMAN antibody
      • Nidogen-2 antibody
      • Nidogen2 antibody
      • Osteonidogen antibody
      see all

    Anti-Nidogen 2 antibody (ab14513) 使用論文

    This product has been referenced in:
    • Hummitzsch K  et al. A new model of development of the mammalian ovary and follicles. PLoS One 8:e55578 (2013). ICC/IF ; Cow . Read more (PubMed: 23409002) »
    • Behrens DT  et al. The epidermal basement membrane is a composite of separate laminin- or collagen IV-containing networks connected by aggregated perlecan, but not by nidogens. J Biol Chem 287:18700-9 (2012). Read more (PubMed: 22493504) »

    See all 4 Publications for this product

    ab14513 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

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    ab14513 の abreview を投稿する ab14513 に関する問い合わせをする

    2 Abreviews or Q&A

    10
    Votes: Highest First

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Nidogen 2 antibody

     Average
    Abreviews
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (retinal tissue)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Heat mediated antigen retrieval solution pH: 6
    Permeabilization Yes - 0.1% Tween 20
    Specification retinal tissue
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative Metanol 95% C/ Acético Glacial 5%
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    Dr. Patricia Bogdanov

    Verified customer

    投稿 Jul 18 2016

    Dear technical officer, Here I am writing to enquire about the concentration of the following 2 antibodies (rabbit anti-nidogen1 (Ab14511) and rabbit anti-nidogen2 (Ab14513)as I will need the information to determine the concentration for the isotype c...

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    Thank you for your enquiry. I can confirm that both ab14511 and ab14513 are sold as whole antiserum. As antibody concentration is usually determined by a protein assay, I am sorry it is not possible to provide a concentration for antibody in antiser...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"