製品の概要

  • 製品名
    Anti-Nidogen 2 antibody
    Nidogen 2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Nidogen 2
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: WB, ELISA, IHC-Fr, Flow Cyt, ICC/IFmore details
  • 種交差性
    交差種: Mouse
  • 免疫原

    Recombinant full length protein (Mouse).

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab14513 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent concentration. Predicted molecular weight: 153 kDa.
ELISA Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. PubMed: 23211522

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 
ICC/IF 1/200. PubMed: 23409002

ターゲット情報

  • 機能
    Cell adhesion glycoprotein which is widely distributed in basement membranes. Binds to collagens I and IV, to perlecan and to laminin 1. Does not bind fibulins. It probably has a role in cell-extracellular matrix interactions.
  • 組織特異性
    Heart, placenta and bone. Less in pancreas, kidney and skeletal muscle.
  • 配列類似性
    Contains 5 EGF-like domains.
    Contains 5 LDL-receptor class B repeats.
    Contains 1 NIDO domain.
    Contains 1 nidogen G2 beta-barrel domain.
    Contains 2 thyroglobulin type-1 domains.
  • 翻訳後修飾
    Highly N- and O-glycosylated.
  • 細胞内局在
    Secreted > extracellular space > extracellular matrix > basement membrane.
  • Information by UniProt
  • 参照データベース
    • 別名
      • NID 2 antibody
      • NID-2 antibody
      • Nid2 antibody
      • NID2_HUMAN antibody
      • Nidogen-2 antibody
      • Nidogen2 antibody
      • Osteonidogen antibody
      see all

    参考文献

    This product has been referenced in:
    • Murphy PA  et al. Tumor angiogenesis in the absence of fibronectin or its cognate integrin receptors. PLoS One 10:e0120872 (2015). Read more (PubMed: 25807551) »
    • Torricelli AA  et al. Epithelial basement membrane proteins perlecan and nidogen-2 are up-regulated in stromal cells after epithelial injury in human corneas. Exp Eye Res 134:33-8 (2015). Read more (PubMed: 25797478) »

    See all 6 Publications for this product

    ab14513 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    レビューと Q&A

    レビューを送る 質問を送る

    2 reviews or Q&A

    10
    Votes: Highest First

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Nidogen 2 antibody

     Average
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (retinal tissue)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Heat mediated antigen retrieval solution pH: 6
    Permeabilization
    Yes - 0.1% Tween 20
    Specification
    retinal tissue
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    Metanol 95% C/ Acético Glacial 5%
    Read More
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    Username

    Dr. Patricia Bogdanov

    Verified customer

    投稿 Jul 18 2016

    Dear technical officer, Here I am writing to enquire about the concentration of the following 2 antibodies (rabbit anti-nidogen1 (Ab14511) and rabbit anti-nidogen2 (Ab14513)as I will need the information to determine the concentration for the isotype c...

    Read More

    Thank you for your enquiry. I can confirm that both ab14511 and ab14513 are sold as whole antiserum. As antibody concentration is usually determined by a protein assay, I am sorry it is not possible to provide a concentration for antibody in antiser...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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