Anti-Nicotinic Acetylcholine Receptor beta/CHRNB1 抗体 [EP2067Y] (ab76159)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP2067Y] to Nicotinic Acetylcholine Receptor beta/CHRNB1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Nicotinic Acetylcholine Receptor beta/CHRNB1 antibody [EP2067Y]
Nicotinic Acetylcholine Receptor beta/CHRNB1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP2067Y] to Nicotinic Acetylcholine Receptor beta/CHRNB1 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: Flow Cyt,ICC/IF,IHC-P or IP -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: Human, mouse and rat brain lysates.
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特記事項
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP2067Y -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab76159の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/2000. Predicted molecular weight: 57 kDa.
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特記事項 |
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WB
1/1000 - 1/2000. Predicted molecular weight: 57 kDa. |
ターゲット情報
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機能
After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. -
関連疾患
Defects in CHRNB1 are a cause of congenital myasthenic syndrome slow-channel type (SCCMS) [MIM:601462]. SCCMS is the most common congenital myasthenic syndrome. Congenital myasthenic syndromes are characterized by muscle weakness affecting the axial and limb muscles (with hypotonia in early-onset forms), the ocular muscles (leading to ptosis and ophthalmoplegia), and the facial and bulbar musculature (affecting sucking and swallowing, and leading to dysphonia). The symptoms fluctuate and worsen with physical effort. SCCMS is caused by kinetic abnormalities of the AChR, resulting in prolonged endplate currents and prolonged AChR channel opening episodes.
Defects in CHRNB1 are a cause of congenital myasthenic syndrome with acetylcholine receptor deficiency (ACHRDCMS) [MIM:608931]. ACHRDCMS is a post-synaptic congenital myasthenic syndrome. Mutations underlying AChR deficiency cause a 'loss of function' and show recessive inheritance. -
配列類似性
Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Beta-1/CHRNB1 sub-subfamily. -
細胞内局在
Cell junction > synapse > postsynaptic cell membrane. Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 1140 Human
- Entrez Gene: 11443 Mouse
- Entrez Gene: 24261 Rat
- GenBank: NP_000738.2 Human
- Omim: 100710 Human
- SwissProt: P11230 Human
- SwissProt: P09690 Mouse
- SwissProt: P25109 Rat
see all -
別名
- Acetylcholine receptor protein beta chain precursor antibody
- Acetylcholine receptor subunit beta antibody
- ACHB_HUMAN antibody
see all
画像
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Anti-Nicotinic Acetylcholine Receptor beta/CHRNB1 antibody [EP2067Y] (ab76159) at 1/1000 dilution + Human brain lysate at 10 µg
Secondary
Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 57 kDa
Observed band size: 57 kDa -
Anti-Nicotinic Acetylcholine Receptor beta/CHRNB1 antibody [EP2067Y] (ab76159) at 1/1000 dilution + Mouse brain lysate
Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 57 kDa
Observed band size: 57 kDa
Exposure time: 5 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST
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Anti-Nicotinic Acetylcholine Receptor beta/CHRNB1 antibody [EP2067Y] (ab76159) at 1/1000 dilution + Rat brain lysate
Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 57 kDa
Observed band size: 57 kDa
Exposure time: 5 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab76159 は 2 報の論文で使用されています。
- Li B et al. Stability of motor endplates is greater in the biceps than in the interossei in a rat model of obstetric brachial plexus palsy. Neural Regen Res 15:1678-1685 (2020). PubMed: 32209772
- Kelly NA et al. Effects of aging and Parkinson's disease on motor unit remodeling: influence of resistance exercise training. J Appl Physiol (1985) 124:888-898 (2018). PubMed: 29357501