Anti-Nicotinic Acetylcholine Receptor beta 4 抗体 (ab41176)

製品の概要

  • 製品名Anti-Nicotinic Acetylcholine Receptor beta 4 antibody
    Nicotinic Acetylcholine Receptor beta 4 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Nicotinic Acetylcholine Receptor beta 4
  • 特異性80 % cross reactivity with the full lenght Nicotinic ß4 receptor
  • アプリケーション適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide:

    EGPYAAQRD

    , corresponding to C terminal amino acids 490-498 of Human Nicotinic Acetylcholine Receptor beta 4 attached onto a carrier protein.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab41176 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/200. Detects a band of approximately 60 kDa (predicted molecular weight: 56 kDa). With this antibody, we have found that blocking with 5% goat or donkey serum significantly reduces background as compared to BSA or milk.
ICC/IF 1/200.

ターゲット情報

  • 機能After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
  • 配列類似性Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Beta-4/CHRNB4 sub-subfamily.
  • 細胞内局在Cell junction > synapse > postsynaptic cell membrane. Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • acetylcholine receptor, nicotinic, beta 4 (neuronal) antibody
    • ACHB4_HUMAN antibody
    • AChR antibody
    • Cholinergic receptor nicotinic beta 4 antibody
    • Cholinergic receptor nicotinic beta polypeptide 4 antibody
    • cholinergic receptor, nicotinic, beta polypeptide 4 antibody
    • Chrnb4 antibody
    • Neuronal acetylcholine receptor subunit beta-4 antibody
    • Neuronal nicotinic receptor beta 4 subunit antibody
    see all

Anti-Nicotinic Acetylcholine Receptor beta 4 antibody 画像

  • ICC/IF image of ab41176 stained SKNSH cells. The cells were 4% PFA fixed (10mins) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab41176, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Nicotinic Acetylcholine Receptor beta 4 antibody (ab41176) 使用論文

ab41176 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab41176.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"