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Recombinant fragment (Chicken): Bacterially-expressed large cytoplasmic domain of Nicotinic Acetylcholine Receptor alpha 7.
Our Abpromise guarantee covers the use of ab24644 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab24644 staining Nicotinic Acetylcholine Receptor alpha 7 in rat hippocampus tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin embedded sections). Sections were PFA-fixed and subjected to antigen retrieval by autoclave prior to blocking with 8% milk for 30 minutes at 37°C. The primary antibody was diluted 1/50 with DAKO antibody diluent and incubated with the sample for 18 hours at 4°C. An undiluted HRP-conjugated Goat anti-rat polyclonal was used as the secondary antibody.