Anti-Nicotinic Acetylcholine Receptor alpha 5 抗体 (ab118138)

製品の概要

  • 製品名Anti-Nicotinic Acetylcholine Receptor alpha 5 antibody
    Nicotinic Acetylcholine Receptor alpha 5 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to Nicotinic Acetylcholine Receptor alpha 5
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee, Macaque Monkey
  • 免疫原

    Synthetic peptide:

    HVDRYFTQKEET

    with a Cysteine residue linker, corresponding to internal sequence amino acids 369-380 of Human Nicotinic Acetylcholine Receptor alpha 5 (NP_000736.2).

  • ポジティブ・コントロール
    • Kelly (Human neuroblastoma cell line) lysate.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファーpH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)ab118138 is purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab118138 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 53 kDa).

ターゲット情報

  • 機能After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
  • 配列類似性Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha-5/CHRNA5 sub-subfamily.
  • 細胞内局在Cell junction > synapse > postsynaptic cell membrane. Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Acetylcholine receptor nicotinic alpha 5 (neuronal) antibody
    • ACHA5_HUMAN antibody
    • AChR antibody
    • Cholinergic receptor neuronal nicotinic alpha polypeptide 5 antibody
    • Cholinergic receptor nicotinic alpha 5 antibody
    • Cholinergic receptor nicotinic alpha polypeptide 5 antibody
    • CHRNA 5 antibody
    • CHRNA5 antibody
    • LNCR2 antibody
    • NACHRA 5 antibody
    • NACHRA5 antibody
    • Neuronal acetylcholine receptor subunit alpha 5 antibody
    • Neuronal acetylcholine receptor subunit alpha-5 antibody
    • Neuronal nicotinic acetylcholine receptor alpha 5 subunit antibody
    • Neuronal nicotinic acetylcholine receptor, alpha5 subunit antibody
    • OTTHUMP00000185019 antibody
    see all

Anti-Nicotinic Acetylcholine Receptor alpha 5 antibody 画像

  • Anti-Nicotinic Acetylcholine Receptor alpha 5 antibody (ab118138) at 1 µg/ml (for 1 hour) + Kelly cell lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 53 kDa

Anti-Nicotinic Acetylcholine Receptor alpha 5 antibody (ab118138) 使用論文

ab118138 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (ventral tegmental area (VTA) and cortex)
Loading amount 10 µg
Specification ventral tegmental area (VTA) and cortex
Treatment RIPA(1X)/EDTA(0.05M)/cocktail protease inhibitor, benzonase 37°C for 20min and ice for 30 min
Gel Running Conditions Reduced Denaturing (4-12% BIS-TRIS)
Blocking step Milk as blocking agent for 6 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Apr 02 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (ventral tegmental area (VTA))
Loading amount 10 µg
Specification ventral tegmental area (VTA)
Treatment RIPA(1X)/EDTA(0.05M)/cocktail protease inhibitor, benzonase 37°C for 20min and ice for 30 min
Gel Running Conditions Reduced Denaturing (4-12% BIS-TRIS)
Blocking step Milk as blocking agent for 6 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Mar 29 2013

Code de remise : *****
Date d’expiration : JJ MM AAAA

Comme convenu hier par téléphone j'ai comparé la séquence immunogène du ab118138 avec les protéines Nicotinic Acetylcholine Receptor alph...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"