Anti-Nicotinic Acetylcholine Receptor alpha 4 抗体 (ab77356)

製品の概要

  • 製品名Anti-Nicotinic Acetylcholine Receptor alpha 4 antibody
    Nicotinic Acetylcholine Receptor alpha 4 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to Nicotinic Acetylcholine Receptor alpha 4
  • 特異性ab77356 is not expected to cross-react with the similar alpha 2 subunit.
  • アプリケーション適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Rat, Human
    交差が予測される動物種: Mouse, Chicken, Cow, Dog, Chimpanzee
  • 免疫原

    Synthetic peptide:

    HVETRAHAEERLLKK

    , corresponding to internal sequence amino acids 29-43 of Human Nicotinic Acetylcholine Receptor alpha 4

  • ポジティブ・コントロール
    • Rat Brain lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab77356 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA
WB
  • 追加情報Peptide ELISA: Use at an assay dependent dilution. Antibody detection limit dilution 1/64,000.
    WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).
    An additional band of unknown identity was also consistently observed at 22kDa. This band was successfully blocked by incubation with the immunising peptide.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
    • 関連疾患Defects in CHRNA4 are the cause of nocturnal frontal lobe epilepsy type 1 (ENFL1) [MIM:600513]; also symbolized ADNFLE. ENFL1 is an autosomal dominant epilepsy characterized by nocturnal seizures with hyperkinetic automatisms and poorly organized stereotyped movements.
    • 配列類似性Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha-4/CHRNA4 sub-subfamily.
    • 細胞内局在Cell junction > synapse > postsynaptic cell membrane. Cell membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • A4 nicotinic receptor antibody
      • Acetylcholine receptor alpha 4 neural antibody
      • Acetylcholine receptor neuronal nicotinic alpha 4 subunit antibody
      • ACH 4 antibody
      • ACH4 antibody
      • ACHA4_HUMAN antibody
      • AChR antibody
      • Acra 4 antibody
      • Acra4 antibody
      • Alpha4 nAChR antibody
      • BFNC antibody
      • Cholinergic receptor nicotinic alpha 4 antibody
      • Cholinergic receptor nicotinic alpha polypeptide 4 antibody
      • CHRNA 4 antibody
      • CHRNA4 antibody
      • EBN 1 antibody
      • EBN antibody
      • EBN1 antibody
      • NACHR antibody
      • NACRA 4 antibody
      • NACRA4 antibody
      • Neuronal acetylcholine receptor subunit alpha-4 antibody
      • Neuronal nicotinic acetylcholine receptor alpha 4 subunit antibody
      see all

    Anti-Nicotinic Acetylcholine Receptor alpha 4 antibody 画像

    • Anti-Nicotinic Acetylcholine Receptor alpha 4 antibody (ab77356) at 0.3 µg/ml + Rat Brain lysate (in RIPA buffer) at 35 µg
      Developed using the ECL technique

      Predicted band size : 70 kDa
      Observed band size : 70 kDa
      Additional bands at : 22 kDa. We are unsure as to the identity of these extra bands.

    Anti-Nicotinic Acetylcholine Receptor alpha 4 antibody (ab77356) 使用論文

    ab77356 has not yet been referenced specifically in any publications.

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    Thank you for your inquiry.

    The molecular weight of the peptide was not determined.

    The molecular weight of the antibody is ca 150kDa.

    Molecular weight is not necessary to calculate the dilutions of the blocking peptide. C...

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    Vielen Dank für Ihren Anruf. Auf Ihre Anfrage hin haben wir das Blockpeptid für den Acetylcholin-Rezeptor alpha 4 (ab77356) in unseren Katalog aufgenommen. Es hat die Artikelnummer ab123011 und kostet 175 EUR plus Versandgebühren. Click here (or ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"