Anti-Nicotinic Acetylcholine Receptor alpha 2 抗体 (ab48943)

製品の概要

  • 製品名
    Anti-Nicotinic Acetylcholine Receptor alpha 2 antibody
    Nicotinic Acetylcholine Receptor alpha 2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Nicotinic Acetylcholine Receptor alpha 2
  • アプリケーション
    適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee
  • 免疫原

    A region within synthetic peptide: MGPSCPVFLS FTKLSLWWLL LTPAGGEEAK RPPPRAPGDP LSSPSPTALP, corresponding to N terminal amino acids 1-50 of Human Nicotinic Acetylcholine Receptor alpha 2

  • ポジティブ・コントロール
    • HepG2 cell lysates Raji cell lysates

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度
    Protein A purified
  • 特記事項(精製)
    Purified by Protein G/A affinity chromatography method.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab48943 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/15000.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 60 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能
    After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
  • 関連疾患
    Defects in CHRNA2 are the cause of nocturnal frontal lobe epilepsy type 4 (ENFL4) [MIM:610353]. ENFL4 is an autosomal dominant epilepsy characterized by nocturnal seizures associated with fear sensation, tongue movements, and nocturnal wandering, closely resembling nightmares and sleep walking.
  • 配列類似性
    Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha-2/CHRNA2 sub-subfamily.
  • 細胞内局在
    Cell junction > synapse > postsynaptic cell membrane. Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ACHA2_HUMAN antibody
    • AChR antibody
    • Cholinergic receptor nicotinic alpha 2 antibody
    • Chrna2 antibody
    • Neuronal acetylcholine receptor protein subunit alpha 2 antibody
    • Neuronal acetylcholine receptor subunit alpha-2 antibody
    see all

Anti-Nicotinic Acetylcholine Receptor alpha 2 antibody 画像

  • All lanes : Anti-Nicotinic Acetylcholine Receptor alpha 2 antibody (ab48943) at 2.5 µg/ml

    Lane 1 : HepG2 cell lysate
    Lane 2 : Raji cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution


    Predicted band size : 60 kDa
    Observed band size : 58 kDa (why is the actual band size different from the predicted?)

プロトコール

Anti-Nicotinic Acetylcholine Receptor alpha 2 antibody (ab48943) 使用論文

ab48943 has not yet been referenced specifically in any publications.

Product Wall

Application
Western blot
Sample
Mouse Cell lysate - whole cell (primary hepatocyte)
Gel Running Conditions
Reduced Denaturing (8 %)
Loading amount
40 µg
Specification
primary hepatocyte
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Mr. Dongil Kim

Verified customer

投稿 Oct 20 2015

Thank you for providing further information. As we had not previously test this antibody in mouse tissue we do not know what type of banding pattern you would receive. Based on the information that I have found, the mouse version of Nicotinic Acetylcho...

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I will happily send you a positive control if that is what is needed to verify the problem. Before I do that, I do want to make sure that sending a positive control is what is needed and not a replacement antibody. Could you provide me the answers to t...

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I am sorry that you are experiencing problems using ab48943 in western blot. To better understand the problems that you are having with the antibody would it be possible for you to provide a copy of the protocol that you are using (total protein loa...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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