製品の概要
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製品名Anti-Nicastrin antibody
Nicastrin 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to Nicastrin
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由来種Rabbit
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アプリケーション適用あり: WB, IHC-P, ICC/IFmore details
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種交差性交差種: Mouse, Human
交差が予測される動物種: Pig, Macaque monkey, Gorilla, Orangutan
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免疫原
Synthetic peptide conjugated to KLH derived from within residues 450 - 550 of Human Nicastrin.
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ポジティブ･コントロール
- This antibody gave a positive signal in the following tissue lysates: E10 Mouse Embryonic Brain; E12 Mouse Embryonic Brain and Spinal Cord; E16 Mouse Embryonic Brain. ICC/IF: Mouse Embryonic Stem cells.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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バッファーpH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab122969 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 120 kDa (predicted molecular weight: 78 kDa). | |
| IHC-P | 1/8000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| ICC/IF | Use a concentration of 5 µg/ml. |
ターゲット情報
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機能Essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). It probably represents a stabilizing cofactor required for the assembly of the gamma-secretase complex.
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組織特異性Widely expressed.
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配列類似性Belongs to the nicastrin family.
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細胞内局在Membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
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参照データベース
- Entrez Gene: 101134867 Gorilla
- Entrez Gene: 23385 Human
- Entrez Gene: 59287 Mouse
- Entrez Gene: 100172503 Orangutan
- Omim: 605254 Human
- SwissProt: Q92542 Human
- SwissProt: P57716 Mouse
- Unigene: 517249 Human
see all -
別名
- Anterior pharynx defective 2 antibody
- APH 2 antibody
- APH2 antibody
see all
画像
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Western blot - Anti-Nicastrin antibody (ab122969)All lanes : Anti-Nicastrin antibody (ab122969) at 1 µg/ml
Lane 1 : E10 Mouse Embryo Brain Tissue Lysate
Lane 2 : E12 Mouse Embryo Brain and Spinal Cord Tissue Lysate
Lane 3 : E16 Mouse Embryo Brain Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 78 kDa
Observed band size: 120 kDa (why is the actual band size different from the predicted?)
Additional bands at: 75 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
Nicastrin contains an exstensive number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. -
Immunocytochemistry/ Immunofluorescence - Anti-Nicastrin antibody (ab122969)ICC/IF image of ab122969 stained Mouse Embryonic Stem cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab122969 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicastrin antibody (ab122969)This image is courtesy of an Abreview submitted by Carl Hobbsab122969 staining Nicastrin in Human breast carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/8000 in TBS/BSA/azide) for 16 hours at 21°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/250) was used as the secondary antibody.
プロトコール
参考文献
This product has been referenced in:
- Xu TH et al. Alzheimer's disease-associated mutations increase amyloid precursor protein resistance to ?-secretase cleavage and the Aß42/Aß40 ratio. Cell Discov 2:16026 (2016). WB ; Human . Read more (PubMed: 27625790) »
レビューと Q&A
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