Synthetic peptide conjugated to KLH derived from within residues 450 - 550 of Human Nicastrin.
Our Abpromise guarantee covers the use of ab122969 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB | Use a concentration of 1 µg/ml. Detects a band of approximately 120 kDa (predicted molecular weight: 78 kDa). | |
IHC-P | 1/8000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
ICC/IF | Use a concentration of 5 µg/ml. |
ICC/IF image of ab122969 stained Mouse Embryonic Stem cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab122969 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"