Anti-NHP2L1 抗体 (ab95958)
Key features and details
- Rabbit polyclonal to NHP2L1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-NHP2L1 antibody
NHP2L1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to NHP2L1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Zebrafish -
免疫原
Recombinant fragment corresponding to Human NHP2L1 aa 1-98.
Database link: P55769 -
ポジティブ・コントロール
- WB: HeLa and HepG2 whole cell lysates ICC/IF: Hela cell IHC-P: Cal27 xenograft
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab95958の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (2) |
1/500 - 1/3000. Predicted molecular weight: 14 kDa.
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IHC-P |
1/100 - 1/250.
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ICC/IF | (1) |
1/100 - 1/200.
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特記事項 |
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WB
1/500 - 1/3000. Predicted molecular weight: 14 kDa. |
IHC-P
1/100 - 1/250. |
ICC/IF
1/100 - 1/200. |
ターゲット情報
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関連性
Originally named because of its sequence similarity to the Saccharomyces cerevisiae NHP2 (non histone protein 2), this protein appears to be a highly conserved nuclear protein that is a component of the [U4/U6.U5] tri snRNP. It binds to the 5' stem loop of U4 snRNA. Two transcript variants encoding the same protein have been found for this gene. -
細胞内局在
Nucleus › nucleolus. Note: Concentrated in the dense fibrillar component of the nucleolus. -
参照データベース
- Entrez Gene: 4809 Human
- Entrez Gene: 321114 Zebrafish
- Omim: 601304 Human
- SwissProt: P55769 Human
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別名
- [U4/U6.U5] tri snRNP antibody
- 15.5 kD RNA binding protein antibody
- High mobility group like nuclear protein 2 homolog 1 antibody
see all
画像
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All lanes : Anti-NHP2L1 antibody (ab95958) at 1/1000 dilution
Lane 1 : Hela whole cell lysate
Lane 2 : HepG2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 14 kDa
12% SDS PAGE -
ab95958, at a 1/200 dilution, staining NHP2L1 in paraformaldehyde fixed HeLa cell by Immunofluorescence analysis.
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ab95958, at a 1/100 dilution, staining NHP2L1 in paraffin embedded Cal27 xenograft by Immunohistochemistry.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab95958 は 5 報の論文で使用されています。
- Koedoot E et al. Splicing factors control triple-negative breast cancer cell mitosis through SUN2 interaction and sororin intron retention. J Exp Clin Cancer Res 40:82 (2021). PubMed: 33648524
- Diouf B et al. Identification of small molecules that mitigate vincristine-induced neurotoxicity while sensitizing leukemia cells to vincristine. Clin Transl Sci 14:1490-1504 (2021). PubMed: 33742760
- Falaleeva M et al. Dual function of C/D box small nucleolar RNAs in rRNA modification and alternative pre-mRNA splicing. Proc Natl Acad Sci U S A 113:E1625-34 (2016). PubMed: 26957605
- Tremblay N et al. Spliceosome SNRNP200 Promotes Viral RNA Sensing and IRF3 Activation of Antiviral Response. PLoS Pathog 12:e1005772 (2016). WB . PubMed: 27454487
- Sundaramoorthy S et al. Functional genomics identifies a requirement of pre-mRNA splicing factors for sister chromatid cohesion. EMBO J N/A:N/A (2014). WB . PubMed: 25257310