製品の概要

  • 製品名Anti-NHEDC1 antibody
    NHEDC1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to NHEDC1
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 1-50 (MHTTESKNEH LEDENFQTST TPQSLIDPNN TAHEETKTVL SDTEEIKPQT) of Human NHEDC1 (NP_001094344).

  • ポジティブ・コントロール
    • Human fetal brain cell lysate

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab106124 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.5 µg/ml. Predicted molecular weight: 56 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 組織特異性Expressed only in the testis.
  • 配列類似性Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 1700094G20Rik antibody
    • 4933424B12Rik antibody
    • 4933425K02Rik antibody
    • AV258602 antibody
    • MGC131641 antibody
    • Na(+)/H(+) exchanger-like domain-containing protein 1 antibody
    • NHDC1_HUMAN antibody
    • NHE domain-containing protein 1 antibody
    • Nhedc1 antibody
    • Sodium/hydrogen exchanger-like domain-containing protein 1 antibody
    see all

Anti-NHEDC1 antibody 画像

  • Anti-NHEDC1 antibody (ab106124) at 0.5 µg/ml + Human fetal brain cell lysate at 10 µg

    Predicted band size : 56 kDa

Anti-NHEDC1 antibody (ab106124) 使用論文

ab106124 has not yet been referenced specifically in any publications.

Product Wall

Thank you for keeping us updated on your results. I am sorry to hear that the secondary antibody is gives non-specific bands. I'm assuming that you ran a blot without the primary antibody, and that is how you know the extra bands are from the secondary...

Read More

Thank you for your reply. Regarding the sample buffer, we used the following:  0.05M Tris-HCl, pH6.8, 0.1M DTT, 2%SDS, 10% glycerol, 0.1% bromophenol blue Although slightly, different, your buffer would be fine as well. Samples were then heated...

Read More

Thank you for contacting Abcam regarding ab106124. Please find the WB protocol used to validate this antibody below: - Prepare 12% gel - Load 10ug human fetal brain cell lysate - Transfer protein to PVDF membrane (we used Bio-Rad Trans-Blot Ce...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"