製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to NHEDC1
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 1-50 (MHTTESKNEH LEDENFQTST TPQSLIDPNN TAHEETKTVL SDTEEIKPQT) of Human NHEDC1 (NP_001094344).

  • ポジティブ・コントロール
    • Human fetal brain cell lysate

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab106124 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.5 µg/ml. Predicted molecular weight: 56 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 組織特異性
    Expressed only in the testis.
  • 配列類似性
    Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family.
  • 細胞内局在
    Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 1700094G20Rik antibody
    • 4933424B12Rik antibody
    • 4933425K02Rik antibody
    • AV258602 antibody
    • MGC131641 antibody
    • Na(+)/H(+) exchanger-like domain-containing protein 1 antibody
    • NHDC1_HUMAN antibody
    • NHE domain-containing protein 1 antibody
    • Nhedc1 antibody
    • Sodium/hydrogen exchanger-like domain-containing protein 1 antibody
    see all

Anti-NHEDC1 antibody 画像

  • Anti-NHEDC1 antibody (ab106124) at 0.5 µg/ml + Human fetal brain cell lysate at 10 µg

    Predicted band size : 56 kDa

プロトコール

Anti-NHEDC1 antibody (ab106124) 使用論文

This product has been referenced in:
  • Kumar PL & James PF Identification and characterization of methylation-dependent/independent DNA regulatory elements in the human SLC9B1 gene. Gene 561:235-48 (2015). Read more (PubMed: 25701605) »

See 1 Publication for this product

Product Wall

Thank you for keeping us updated on your results. I am sorry to hear that the secondary antibody is gives non-specific bands. I'm assuming that you ran a blot without the primary antibody, and that is how you know the extra bands are from the secondary...

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Thank you for your reply. Regarding the sample buffer, we used the following:  0.05M Tris-HCl, pH6.8, 0.1M DTT, 2%SDS, 10% glycerol, 0.1% bromophenol blue Although slightly, different, your buffer would be fine as well. Samples were then heated...

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Thank you for contacting Abcam regarding ab106124. Please find the WB protocol used to validate this antibody below: - Prepare 12% gel - Load 10ug human fetal brain cell lysate - Transfer protein to PVDF membrane (we used Bio-Rad Trans-Blot Ce...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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