製品の概要

  • 製品名Anti-NGF antibody [EP1318Y]
    NGF 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP1318Y] to NGF
  • 特異性This antibody recognizes pro-NGF.
  • アプリケーション適用あり: WB, Flow Cytmore details
    適用なし: IHC-P
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues near the N-terminus of human NGF-beta precursor (pro-NGF).

  • ポジティブ・コントロール
    • Human pro-NGF recombinant protein
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels.

    If you have any questions regarding this update, please contact our Scientific Support team.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab68151 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Predicted molecular weight: 27 kDa.

For unpurified use at 1/1000 - 1/5000.

Flow Cyt 1/200 - 1/280.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • 追加情報Is unsuitable for IHC-P.
  • ターゲット情報

    • 機能Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival. Inhibits metalloproteinase dependent proteolysis of platelet glycoprotein VI (PubMed:20164177).
    • 関連疾患Neuropathy, hereditary sensory and autonomic, 5
    • 配列類似性Belongs to the NGF-beta family.
    • 細胞内局在Secreted.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Beta nerve growth factor antibody
      • Beta NGF antibody
      • Beta-nerve growth factor antibody
      • Beta-NGF antibody
      • HSAN5 antibody
      • MGC161426 antibody
      • MGC161428 antibody
      • Nerve growth factor (beta polypeptide) antibody
      • Nerve growth factor antibody
      • Nerve growth factor beta antibody
      • Nerve growth factor beta polypeptide antibody
      • Nerve growth factor beta subunit antibody
      • NGF antibody
      • NGF_HUMAN antibody
      • NGFB antibody
      • NID67 antibody
      see all

    Anti-NGF antibody [EP1318Y] 画像

    • Overlay histogram showing SH-SY5Y cells stained with ab68151 (red line) at 1/280 dilution. The cells were fixed with 2% paraformaldehyde. The secondary antibody used was a FITC conjugated goat anti-rabbit IgG at 1/150 dilution. Isotype control antibody (black line) was rabbit monoclonal IgG used under the same conditions. Cells also incubated without primary antibody and secondary antibody (blue line)

    • Anti-NGF antibody [EP1318Y] (ab68151) at 1/1000 dilution + Rat brain lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG, (H+L), HRP-conjugated at 1/1000 dilution

      Predicted band size : 27 kDa
    • Anti-NGF antibody [EP1318Y] (ab68151) at 1/1000 dilution + Human fetal brain lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/1000 dilution

      Predicted band size : 27 kDa
    • All lanes : Anti-NGF antibody [EP1318Y] (ab68151) at 1/5000 dilution (unpurified)

      Lane 1 : 10ng human pro-NGF recombinant protein
      Lane 2 : 5ng human pro-NGF recombinant protein
      Lane 3 : 1ng human pro-NGF recombinant protein
      Lane 4 : 0.5ng human pro-NGF recombinant protein

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 27 kDa
      Observed band size : 27 kDa
      Additional bands at : 13 kDa,20 kDa. We are unsure as to the identity of these extra bands.

    Anti-NGF antibody [EP1318Y] (ab68151) 使用論文

    ab68151 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab68151.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"