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Our Abpromise guarantee covers the use of ab16160 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent dilution.|
|ELISA||Use a concentration of 2 µg/ml. Can be paired for ELISA with Mouse monoclonal [AS21] to NGF (ab16161). Typically for human NGF, the ELISA has a working range of 0-15 ng/ml and a sensitivity of 15 pg/ml with the appropriate standard. Recommended sample volumes are 25-200 µl. Recovery of human NGF in human plasma is typically > 90% in the ELISA. The use of Tris buffer, pH 7, instead of PBS is recommended. Two site ELISA.|
ab16160 has not yet been referenced specifically in any publications.