製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to NGF
  • アプリケーション
    適用あり: Neutralising, WB, ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Highly pure (>98%) recombinant hNGFb (human Nerve Growth Factor beta).

製品の特性

  • 製品の状態
    Lyophilised:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • バッファー
    PBS, pH 7.4, no preservative, sterile filtered
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab9795 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Neutralising
WB
ELISA
  • 追加情報
    ELISA: Use at a concentration of 0.5 µg/ml, allows the detection of 0.2 - 0.4 ng/well of recombinant hNGFb.

    WB: Use at a concentration of 0.1 - 0.2 µg/ml, detection limit for recombinant hNGFb is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

    Neut: To yield one-half maximal inhibition [ND50] of the biological activity of NGF (1 ng/ml), a concentration of 0.05-0.08 µg/ml of this antibody is required.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival. Inhibits metalloproteinase dependent proteolysis of platelet glycoprotein VI (PubMed:20164177).
    • 関連疾患
      Neuropathy, hereditary sensory and autonomic, 5
    • 配列類似性
      Belongs to the NGF-beta family.
    • 細胞内局在
      Secreted.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Beta nerve growth factor antibody
      • Beta NGF antibody
      • Beta-nerve growth factor antibody
      • Beta-NGF antibody
      • HSAN5 antibody
      • MGC161426 antibody
      • MGC161428 antibody
      • Nerve growth factor (beta polypeptide) antibody
      • Nerve growth factor antibody
      • Nerve growth factor beta antibody
      • Nerve growth factor beta polypeptide antibody
      • Nerve growth factor beta subunit antibody
      • NGF antibody
      • NGF_HUMAN antibody
      • NGFB antibody
      • NID67 antibody
      see all

    プロトコール

    参考文献

    This product has been referenced in:
    • Ferrari MP  et al. Safety and pharmacokinetics of escalating doses of human recombinant nerve growth factor eye drops in a double-masked, randomized clinical trial. BioDrugs 28:275-83 (2014). Read more (PubMed: 24327173) »

    See 1 Publication for this product

    レビューと Q&A


    The source of the recombinant immunogen used to produce ab9795 is E. coli.

    Thank you for contacting us. Please find attached the CoC form. At -20C the antibodies are viable for 10 years. At 4C these are viable for 1 year. Please be advised that Abcam guarantee is applicable on products for 6 months on...

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    Thank you for contacting us. We can certainly provide the Certificate of Compliance. Could you send me the lot number of antibody used? Antibodies are stable for years when stored at proper temperature e.g. at -20C the antibodies are via...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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