製品の概要

  • 製品名Anti-NGF antibody
    NGF 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to NGF
  • 特異性Less than 1% cross-reactivity against recombinant human Brain Derived Neurotrophic Factor, Neurotrophin 3 and Neurotrophin 4/5 by ELISA.
  • アプリケーション適用あり: ICC/IF, Dot blot, Neutralising, WB, IHC-FoFr, IHC-Fr, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Chicken, Human
    非交差種: Cow
  • 免疫原

    Native mouse salivary gland NGF purified by modification of the method of Mobley et al (1976). Antigen purity was greater than 95% by PAGE.

  • ポジティブ・コントロール
  • 特記事項This antibody has been shown to be useful for a variety of techniques and its specificity has been demonstrated by immunoblot.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab6199 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 10 µg/ml.
Dot blot Use at an assay dependent concentration.
Neutralising Use a concentration of 2 - 10 µg/ml. 2ug/ml of ab6199 can neutralize 100ng/ml mouse NGF.
WB Use a concentration of 1 µg/ml.
IHC-FoFr 1/300 - 1/5000. (see Abreview on perfusion fixed tissue for detailed protocol).
IHC-Fr Use at an assay dependent concentration.
IHC-P 1/500.

ターゲット情報

  • 機能Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival. Inhibits metalloproteinase dependent proteolysis of platelet glycoprotein VI (PubMed:20164177).
  • 関連疾患Neuropathy, hereditary sensory and autonomic, 5
  • 配列類似性Belongs to the NGF-beta family.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Beta nerve growth factor antibody
    • Beta NGF antibody
    • Beta-nerve growth factor antibody
    • Beta-NGF antibody
    • HSAN5 antibody
    • MGC161426 antibody
    • MGC161428 antibody
    • Nerve growth factor (beta polypeptide) antibody
    • Nerve growth factor antibody
    • Nerve growth factor beta antibody
    • Nerve growth factor beta polypeptide antibody
    • Nerve growth factor beta subunit antibody
    • NGF antibody
    • NGF_HUMAN antibody
    • NGFB antibody
    • NID67 antibody
    see all

Anti-NGF antibody 画像

  • ab6199 staining perfusion fixed rat brain and dorsal root ganglion by IHC-Fr. Animals were pre-perfused with Tris buffer pH 10, followed by 4% paraformadehyde and 15% of a saturated solution of picric acid. The brains were post-fixed in the same fixative overnight, cryoprotected in 20% sucrose for 24 hours, frozen and cut with a cryostat. Free floating immunostaining was performed. An Alexa Fluor ® 488 conjugated goat anti-rat antibody was used as the secondary.

    The image shows the staining obtained with this antibody using direct fluorescence in the rat cortex and dorsal root ganglion. The staining is not only of the cell body of the cortical neurons but a part of their processes.

    See Abreview

  • ab6199 staining NGF in Human tendon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with Dako FLEX Peroxidase blocking for 5 minutes at room temperature; antigen retrieval was by heat mediation in Dako high pH. Samples were incubated with primary antibody (1/250) for 30 minutes. An undiluted HRP-conjugated Goat polyclonal was used as the secondary antibody.

    See Abreview

  • ICC/IF image of ab6199 stained MEF1 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6199, 10µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.


  • Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 30 seconds
  • ab6199 at a 1/500 dilution staining rat brain tissue sections by Immunohistochemistry (Formalin-fixed paraffin-embedded sections). The tissue section was paraformaldehyde fixed and blocked with 2% BSA prior to incubation with the antibody for 24 hours. Bound antibody was detected using a biotinylated goat anti-rabbit IgG antibody.

    This image is courtesy of an Abreview submitted by Grazyna Niewiadomska.

    See Abreview

Anti-NGF antibody (ab6199) 使用論文

This product has been referenced in:
  • Esbona K  et al. COX-2 modulates mammary tumor progression in response to collagen density. Breast Cancer Res 18:35 (2016). IHC-P ; Mouse . Read more (PubMed: 27000374) »
  • Moon HJ  et al. Effects of secreted factors in culture medium of annulus fibrosus cells on microvascular endothelial cells: elucidating the possible pathomechanisms of matrix degradation and nerve in-growth in disc degeneration. Osteoarthritis Cartilage 22:344-54 (2014). ICC/IF ; Human . Read more (PubMed: 24361793) »

See all 11 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Dako FLEX Peroxidase blocking as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: RT°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako High pH
Sample Human Tissue sections (Tendon)
Specification Tendon
Permeabilization No
Fixative Formaldehyde
Username

Abcam user community

Verified customer

投稿 Aug 15 2014

Several different lysates could be used as a positive control. We have used ab6199 with MEF1 cell line in Western blotting. Alternatively it has also been used with rat brain tissue and should also work with mouse or human brain tissue. We have a range...

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Thank you for contacting Abcam.

I beleive the rabbit IgG control below would work very well for your application.

http://www.abcam.com/Rabbit-IgG-ChIP-Grade-ab37415.html



Also, please see the attachment to find o...

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Vielen Dank für Ihre Anfrage.
Bitte geben Sie bei einem Volumen von 500ul Antikörper 500ul Glycerin (Glycerol) dazu.
Das ergibt eine Endkonzentration von 50% Glycerin in 1000ul.
Ich hoffe, der Wetteinsatz ist interessant :-)

Suite à notre conversation téléphonique, je vous envoie la référence de la publication de Emoto où différentes méthodes de démasquage d'antigène ont été testées ...

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Thank you for your inquiry.

The calculated molecular weight of NGF is 27kDa according to UniProt (http://www.uniprot.org/uniprot/P01138).

This molecular weight will be higher when the protein is phosphorylated and glycosylated. Acco...

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Thank you for your enquiry.

Here are answers to your questions:

1.So far we know heat mediated antigen retrieval has worked.

2. citrate buffer pH 6,0

3. Optimal dilution must be determined emperically, but 5ug/ml...

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Thank you for your inquiry about a reference that was mentioned by a customer that submitted an Abreview (http://www.ncbi.nlm.nih.gov/pubmed/7815824)


Unfortunately, we do not have access to the specific article that was mentioned, nor d...

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Merci pour votre réponse et ces précisions.

J'ai de nouveau regardé vos marquages et je validerais personnellement ces résultats. Les deux anticorps montrent un bon marquage spécifique et lescontroles n&eacut...

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Comme expliqué dans mon précédent email, les 2 anticorps peuvent être testés à une dilution légèrement supérieure mais le marquage semble tout à fait correct et spécifique. Le ...

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