製品の概要

  • 製品名Anti-NGF antibody
    NGF 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to NGF
  • 特異性Less than 1% cross-reactivity against recombinant human Brain Derived Neurotrophic Factor, Neurotrophin 3 and Neurotrophin 4/5 by ELISA. This NGF antibody detects a band of approximate molecular weight of 13KDa under denaturing conditions. This corresponds the monomer of beta NGF (as it exists as a dimer in the native state).
  • アプリケーション適用あり: Dot blot, ELISA, IHC-Fr, Neutralising, WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Chicken, Human
    非交差種: Cow
  • 免疫原

    Native mouse salivary gland NGF purified by modification of the method of Mobley et al (1976). Antigen purity was greater than 95% by PAGE.

  • 特記事項This antibody has been shown to be useful for a variety of techniques and its specificity has been demonstrated by immunoblot.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab6198 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Dot blot Use at an assay dependent concentration.
ELISA 1/3000.
IHC-Fr 1/400 - 1/1000.
Neutralising 1/2 - 1/10.
WB 1/400 - 1/1000.
IHC-P 1/400 - 1/1000.

ターゲット情報

  • 機能Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival. Inhibits metalloproteinase dependent proteolysis of platelet glycoprotein VI (PubMed:20164177).
  • 関連疾患Neuropathy, hereditary sensory and autonomic, 5
  • 配列類似性Belongs to the NGF-beta family.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Beta nerve growth factor antibody
    • Beta NGF antibody
    • Beta-nerve growth factor antibody
    • Beta-NGF antibody
    • HSAN5 antibody
    • MGC161426 antibody
    • MGC161428 antibody
    • Nerve growth factor (beta polypeptide) antibody
    • Nerve growth factor antibody
    • Nerve growth factor beta antibody
    • Nerve growth factor beta polypeptide antibody
    • Nerve growth factor beta subunit antibody
    • NGF antibody
    • NGF_HUMAN antibody
    • NGFB antibody
    • NID67 antibody
    see all

Anti-NGF antibody 画像

  • ab6198 at 1/1000 staining perfusion fixed rat brain tissue sections by IHC-Fr. The animals were pre-perfused with Tris buffer pH 10, followed by 4% paraformadehyde and 15% of a saturated solution of picric acid. The brains were post-fixed in the same fixative overnight, cryoprotected in 20% sucrose for 24 hours, frozen and cut with a cryostat. Free floating immunostaining was performed. An Alexa-Fluor ® 488 conjugated goat anti-rabbit antibody was used as the secondary.

    The image shows the staining obtained with this antibody using direct fluorescence in rat cortical neurons, with two different magnifications of acquisition (X10 and X20).

    See Abreview

Anti-NGF antibody (ab6198) 使用論文

This product has been referenced in:
  • Wang L  et al. Tadalafil Promotes the Recovery of Peripheral Neuropathy in Type II Diabetic Mice. PLoS One 11:e0159665 (2016). IHC-P . Read more (PubMed: 27438594) »
  • Messer HG  et al. Inhibition of H3K27me3-specific histone demethylases JMJD3 and UTX blocks reactivation of herpes simplex virus 1 in trigeminal ganglion neurons. J Virol 89:3417-20 (2015). Read more (PubMed: 25552720) »

See all 6 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 50 µg
Gel Running Conditions Non-reduced Non-Denaturing (Native) (12.5% gel)
Sample Rat Tissue lysate - whole (bladder)
Specification bladder
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Aug 06 2013

Thank you for your reply, and I apologize for this inconvenience.

I certainly understand that ab9858 is not as useful to you as ab73680 would be. I can issue a credit to you.

The vial of ab8020 should be arriving shortly, please kee...

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Thank you again for your patience.

I apologize, but ab73680 is out of stock, and it may not be put back into stock for several months as recent lots have not passed quality control testing. Would you be interested in trying another vial of ab...

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Thank you for your reply.

Please keep me updated on any progress. I hope that we hear something from them soon.

Please let me know if you need anything else from me at this time, and I'll let you know if I hear from someone at BioCo...

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Thank you again for your patience.

At this point, I have not heard from Bio-Connect regarding these issues and I've looked through our system and don't see that they've corresponded with anyone else at Abcam. Have you received any update from...

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Thank you for getting back to me and for getting in touch with BioConnect. I will let you know if I don't hear anything from them shortly, and we will figure out the vial of ab9669. I'll also send you a confirmation email after I work everything out wi...

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Thank you for your reply and for confirming that information.

I'm processing the refunds and replacements now. I think I've found your order for ab22048, ab9100, and ab8021, but I am having some trouble finding the order for ab9669. Were thes...

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Thank you for getting back to me with your selections.

For CX3CR1, we do have a couple of other anti-human antibodies that have been tested in IHC on FFPE sections, ab8020 and ab51668-

http://www.abcam.com/CX3CR1-antibody-ab8020.htm...

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Thank you for getting back to me, and I am happy to help resolve this issue. I look forward to hearing from you, and please let me know if you would like any assistance selecting replacements, or if you have any other questions.

Have a great ...

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Thank you for contacting us and letting us know about the trouble with these antibodies.

After reviewing the protocol and images, I agree that these antibodies might not be performing as expected. Our guarantee period generally extends for 6 ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"