製品の概要

  • 製品名
    Anti-NGF antibody
    NGF 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to NGF
  • 由来種
    Goat
  • アプリケーション
    適用あり: ELISA, WB, Neutralisingmore details
  • 種交差性
    交差種: Rat
  • 免疫原

    Recombinant full length rat NGF beta protein expressed in Sf 21 insect cells.

  • ポジティブ･コントロール
    • Tested with human erythroleukemic cells (TF-1) expressing recombinant rat NGF beta.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab10837 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use a concentration of 0.5 - 1 µg/ml.
WB Use a concentration of 0.1 - 0.2 µg/ml. Detects a band of approximately 13.2 kDa.
Neutralising Use a concentration of 0.05 - 0.15 µg/ml.

ターゲット情報

  • 機能
    Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival. Inhibits metalloproteinase dependent proteolysis of platelet glycoprotein VI (PubMed:20164177).
  • 関連疾患
    Neuropathy, hereditary sensory and autonomic, 5
  • 配列類似性
    Belongs to the NGF-beta family.
  • 細胞内局在
    Secreted.
  • Information by UniProt
  • 参照データベース
    • 別名
      • Beta nerve growth factor antibody
      • Beta NGF antibody
      • Beta-nerve growth factor antibody
      • Beta-NGF antibody
      • HSAN5 antibody
      • MGC161426 antibody
      • MGC161428 antibody
      • Nerve growth factor (beta polypeptide) antibody
      • Nerve growth factor antibody
      • Nerve growth factor beta antibody
      • Nerve growth factor beta polypeptide antibody
      • Nerve growth factor beta subunit antibody
      • NGF antibody
      • NGF_HUMAN antibody
      • NGFB antibody
      • NID67 antibody
      see all

    プロトコール

    参考文献

    This product has been referenced in:
    • Huang M  et al. Autophagy mediates proteolysis of NPM1 and HEXIM1 and sensitivity to BET inhibition in AML cells. Oncotarget 7:74917-74930 (2016). Read more (PubMed: 27732946) »

    See 1 Publication for this product

    ab10837 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    レビューと Q&A

    レビューを送る 質問を送る

    1 Review or Q&A

    10
    Votes: Highest First

    I have used an in vitro kit that makes use of E.coli s30 extract. The coding part of the NGFb gene (human) has been inserted in a vector, which results in the overexpression of NGF. The cells we have used are cos-7 cells (monkey) transfected with a vec...

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    Thank you for providing some more useful information about your experimental system. I understand now from your recent e-mail, that you used Cos-7 cells (monkey) transfected with a vector carrying the coding part of the human NGFb gene. I have disc...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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