製品の概要

  • 製品名Anti-NG2 antibody [LHM 2]
    NG2 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [LHM 2] to NG2
  • アプリケーション適用あり: IHC-FoFr, WB, IP, IHC-Fr, Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Tissue/ cell preparation (Human - a 375P cell crude extract).

  • 特記事項The LHM2 antibody is a very good marker for melanoma (>90% stained). LHM2 also stains basal cell carcinomas and subpopulation of basal keratinocytes. LHM2 can be used as a diagnostic panel for immunopathology of malignant melanoma and potential candidates for immunoimaging and immunotherapy of disseminated melanoma. The originators of this antibody report that the MCSP is a cell surface marker specific for epidermal stem cells (J. Invest. Dermatol. in press). Potential applications of the LHM2 antibody include use for separation/isolation of epidermal stem cells, detection of stem cells in skin biopsies or epithelial cell cultures and optimisation of culture conditions to retain epidermal stem cell population in in vitro cultures.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab20156 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-FoFr Use at an assay dependent concentration. PubMed: 21970342
WB Use at an assay dependent concentration. Predicted molecular weight: 240 kDa.
IP Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能Proteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. Binds through its extracellular N-terminus growth factors, extracellular matrix proteases modulating their activity. May regulate MPP16-dependent degradation and invasion of type I collagen participating in melanoma cells invasion properties. May modulate the plasminogen system by enhancing plasminogen activation and inhibiting angiostatin. Functions also as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. May promote retraction fiber formation and cell polarization through Rho GTPase activation. May stimulate alpha-4, beta-1 integrin-mediated adhesion and spreading by recruiting and activating a signaling cascade through CDC42, ACK1 and BCAR1. May activate FAK and ERK1/ERK2 signaling cascades.
  • 組織特異性Detected only in malignant melanoma cells.
  • 配列類似性Contains 15 CSPG (NG2) repeats.
    Contains 2 laminin G-like domains.
  • 翻訳後修飾O-glycosylated; contains glycosaminoglycan chondroitin sulfate which are required for proper localization and function in stress fiber formation (By similarity). Involved in interaction with MMP16 and ITGA4.
    Phosphorylation by PRKCA regulates its subcellular location and function in cell motility.
  • 細胞内局在Apical cell membrane. Cell projection > lamellipodium membrane. Localized at the apical plasma membrane it relocalizes to the lamellipodia of astrocytoma upon phosphorylation by PRKCA. Localizes to the retraction fibers. Localizes to the plasma membrane of oligodendrocytes.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 4732461B14Rik antibody
    • AN2 antibody
    • AN2 proteoglycan antibody
    • Chondroitin sulfate proteoglycan 4 (melanoma-associated) antibody
    • Chondroitin sulfate proteoglycan 4 antibody
    • Chondroitin sulfate proteoglycan NG2 antibody
    • Cspg4 antibody
    • Cspg4 chondroitin sulfate proteoglycan 4 antibody
    • CSPG4_HUMAN antibody
    • HMW-MAA antibody
    • HSN tumor-specific antigen antibody
    • Kiaa4232 antibody
    • MCSP antibody
    • MCSPG antibody
    • MEL-CSPG antibody
    • Melanoma chondroitin sulfate proteoglycan antibody
    • Melanoma-associated chondroitin sulfate proteoglycan antibody
    • MELCSPG antibody
    • MSK16 antibody
    • NG2 antibody
    see all

Anti-NG2 antibody [LHM 2] (ab20156) 使用論文

This product has been referenced in:
  • Loibl M  et al. Direct cell-cell contact between mesenchymal stem cells and endothelial progenitor cells induces a pericyte-like phenotype in vitro. Biomed Res Int 2014:395781 (2014). ICC/IF . Read more (PubMed: 24563864) »
  • Somaiah N  et al. The relationship between homologous recombination repair and the sensitivity of human epidermis to the size of daily doses over a 5-week course of breast radiotherapy. Clin Cancer Res 18:5479-88 (2012). IHC-P ; Human . Read more (PubMed: 22855580) »

See all 10 Publications for this product

Product Wall

Thank you for your inquiry.

Indeed, you are right. The three antibodies, ab78284, ab83508 and ab20156, are the only antibodies against NG2 which have been tested in flow cytometry and on human cells. Of those three, the ab20156 is the only on...

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Thank you for your email and for your telephone enquiry earlier today.

As discussed on the telephone, PDGF receptor beta is a membrane protein, and so I would recommend that detection with ab35270 would not usuallyrequire permeablization. Th...

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DISCOUNT CODE for AB85308: Expiration date: I am very pleased to hear you would like to accept our offer and test ab20156 and/or ab85308 in IHC on rabbit. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem thi...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (skin)
Specification skin
Blocking step BSA as blocking agent for 15 minute(s) · Concentration: 3%
Username

Dr. Sonja Muffler

Verified customer

投稿 Dec 14 2006

The antibody was tested in IHC on keratinocytes in the epidermis in the paper Ghali L et al. Epidermal and hair follicle progenitor cells express melanoma-associated chondroitin sulfate proteoglycan core protein. J Invest Dermatol 122:433-42 (2004). Pu...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"