製品の概要

  • 製品名Anti-NG2 antibody [HMB45]
    NG2 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [HMB45] to NG2
  • アプリケーション適用あり: IHC-P, WB, IP, ELISA, Flow Cyt, ICC/IF, IHC-FrFlmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Cell membrane preparation from Human malignant melanoma SK-MEL-28.

  • ポジティブ・コントロール
    • Human melanoma tissue. SK-MEL-28 cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab83508 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 2 - 5 µg/ml.
WB Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 251 kDa.
IP Use a concentration of 2 - 5 µg/ml.
ELISA Use a concentration of 0.01 - 0.1 µg/ml.
Flow Cyt 1/10 - 1/100.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ICC/IF Use at an assay dependent concentration. PubMed: 22701703
IHC-FrFl Use at an assay dependent concentration.

ターゲット情報

  • 機能Proteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. Binds through its extracellular N-terminus growth factors, extracellular matrix proteases modulating their activity. May regulate MPP16-dependent degradation and invasion of type I collagen participating in melanoma cells invasion properties. May modulate the plasminogen system by enhancing plasminogen activation and inhibiting angiostatin. Functions also as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. May promote retraction fiber formation and cell polarization through Rho GTPase activation. May stimulate alpha-4, beta-1 integrin-mediated adhesion and spreading by recruiting and activating a signaling cascade through CDC42, ACK1 and BCAR1. May activate FAK and ERK1/ERK2 signaling cascades.
  • 組織特異性Detected only in malignant melanoma cells.
  • 配列類似性Contains 15 CSPG (NG2) repeats.
    Contains 2 laminin G-like domains.
  • 翻訳後修飾O-glycosylated; contains glycosaminoglycan chondroitin sulfate which are required for proper localization and function in stress fiber formation (By similarity). Involved in interaction with MMP16 and ITGA4.
    Phosphorylation by PRKCA regulates its subcellular location and function in cell motility.
  • 細胞内局在Apical cell membrane. Cell projection > lamellipodium membrane. Localized at the apical plasma membrane it relocalizes to the lamellipodia of astrocytoma upon phosphorylation by PRKCA. Localizes to the retraction fibers. Localizes to the plasma membrane of oligodendrocytes.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 4732461B14Rik antibody
    • AN2 antibody
    • AN2 proteoglycan antibody
    • Chondroitin sulfate proteoglycan 4 (melanoma-associated) antibody
    • Chondroitin sulfate proteoglycan 4 antibody
    • Chondroitin sulfate proteoglycan NG2 antibody
    • Cspg4 antibody
    • Cspg4 chondroitin sulfate proteoglycan 4 antibody
    • CSPG4_HUMAN antibody
    • HMW-MAA antibody
    • HSN tumor-specific antigen antibody
    • Kiaa4232 antibody
    • MCSP antibody
    • MCSPG antibody
    • MEL-CSPG antibody
    • Melanoma chondroitin sulfate proteoglycan antibody
    • Melanoma-associated chondroitin sulfate proteoglycan antibody
    • MELCSPG antibody
    • MSK16 antibody
    • NG2 antibody
    see all

Anti-NG2 antibody [HMB45] 画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast cancer tissue sections labelling NG2 with ab83508 at 1/50. 4μm-thick sections were deparaffinized in xylene, dehydrated through three alcohol changes. Endogenous peroxidase activity was quenched with 3% hydrogen peroxide in methanol. Antigen retrieval was performed in 96°C solution of 0.01 mol/L sodium citrate buffer (pH 6.0) for 30 minutes. Peroxidase/DAB, Rabbit/Mouse kit, after visualization, the sections were counterstained with hematoxylin. Hisg NG2 expression.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast cancer tissue sections labelling NG2 with ab83508 at 1/50. 4μm-thick sections were deparaffinized in xylene, dehydrated through three alcohol changes. Endogenous peroxidase activity was quenched with 3% hydrogen peroxide in methanol. Antigen retrieval was performed in 96°C solution of 0.01 mol/L sodium citrate buffer (pH 6.0) for 30 minutes. Peroxidase/DAB, Rabbit/Mouse kit, after visualization, the sections were counterstained with hematoxylin. Low NG2 expression.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast cancer tissue sections labelling NG2 with ab83508 at 1/50. 4μm-thick sections were deparaffinized in xylene, dehydrated through three alcohol changes. Endogenous peroxidase activity was quenched with 3% hydrogen peroxide in methanol. Antigen retrieval was performed in 96°C solution of 0.01 mol/L sodium citrate buffer (pH 6.0) for 30 minutes. Peroxidase/DAB, Rabbit/Mouse kit, after visualization, the sections were counterstained with hematoxylin. Negative NG2 expression.

  • 5 x 106 SK-MEL-28 cells were incubated with 5µg of ab83508, followed by APC-GtxMs IgG incubation.
  • Immunofluorescence analysis of Human adult blood endothelial progenitor cells (left) and umbilical artery smooth muscle cells (right).

    NG2 was stained using ab83508 at 1/200 dilution. Nuclei were stained with DAPI (blue).

Anti-NG2 antibody [HMB45] (ab83508) 使用論文

This product has been referenced in:
  • Pellegrinelli V  et al. Endothelial cells from visceral adipose tissue disrupt adipocyte functions in a three-dimensional setting: partial rescue by angiopoietin-1. Diabetes 63:535-49 (2014). Human . Read more (PubMed: 24130331) »
  • Hsu NC  et al. High chondroitin sulfate proteoglycan 4 expression correlates with poor outcome in patients with breast cancer. Biochem Biophys Res Commun 441:514-8 (2013). IHC-P ; Human . Read more (PubMed: 24177010) »

See all 5 Publications for this product

Product Wall

Application Immunohistochemistry free floating
Sample Mouse Tissue sections (Brain)
Specification Brain
Username

Abcam user community

Verified customer

投稿 Oct 26 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (intestine)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization No
Specification intestine
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 25°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

投稿 Sep 06 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Sample Human Tissue sections (Melanoma)
Specification Melanoma
Permeabilization No
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

投稿 Oct 10 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (Melanoma)
Specification Melanoma
Preparation Cell harvesting/tissue preparation method: Cell scraper. Pulse/rinse with PBS.
Sample buffer: PBS
Fixation Paraformaldehyde
Permeabilization No
Gating Strategy R660 positive cells (APC+). Gate determined by secondary antibody alone.
Username

Dr. Simon Jones

Verified customer

投稿 May 14 2013

Thank you for contacting us.

The ab83508 reacts with human and mouse samples. It was used for IHC on free floating sections (IHC-FrFl) ( Abreview http://www.abcam.com/index.html?datasheet=83508&tab=abreviews&intabreviewid=26915 ) and I...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (spinal cord)
Specification spinal cord
Fixative Paraformaldehyde
Permeabilization Yes - triton 0.5%
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Aug 28 2012

Thank you once againfor your enquiry and for your patience.

I can confirm that we have mouse as a tested and guaranteed species from the IHC-FrFl Abreview published on the datasheet for ab83508.

I am sorry the originator does not ...

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DISCOUNT CODE: 100%ABR-EBVXU


Expiration date: 26/11/2012

I am very pleased to hear you would like to accept our offer and testab83508 in IHC-Fr. This code will give you: 1 free PRIMARY ANTIBODY before the expiration date. To ...

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Thank you very much for your interest in ab83508.

To our knowledge,ab83508 has not been tested in IHC-Fr. Therefore, I can offer a discount off a future purchase if you buyab83508 now, test it inIHC-Fr and submit feedback to us in the form o...

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Thank you for your enquiry regarding ab83508.

This antibody has been tested and characterized for Immunohistochemistry on formalin-fixed paraffin-embedded tissue sections (IHC-P) and on Free Floating samples (IHC-FrFl). We have not specifical...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"