製品の概要

  • 製品名Anti-NG2 antibody
    NG2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to NG2
  • アプリケーション適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 575-625 of Human NG2 (NP_001888.2).

  • ポジティブ・コントロール
    • Hela whole cell lysate.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab86067 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Predicted molecular weight: 251 kDa.
IP Use at 5-10 µg/mg of lysate.

ターゲット情報

  • 機能Proteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. Binds through its extracellular N-terminus growth factors, extracellular matrix proteases modulating their activity. May regulate MPP16-dependent degradation and invasion of type I collagen participating in melanoma cells invasion properties. May modulate the plasminogen system by enhancing plasminogen activation and inhibiting angiostatin. Functions also as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. May promote retraction fiber formation and cell polarization through Rho GTPase activation. May stimulate alpha-4, beta-1 integrin-mediated adhesion and spreading by recruiting and activating a signaling cascade through CDC42, ACK1 and BCAR1. May activate FAK and ERK1/ERK2 signaling cascades.
  • 組織特異性Detected only in malignant melanoma cells.
  • 配列類似性Contains 15 CSPG (NG2) repeats.
    Contains 2 laminin G-like domains.
  • 翻訳後修飾O-glycosylated; contains glycosaminoglycan chondroitin sulfate which are required for proper localization and function in stress fiber formation (By similarity). Involved in interaction with MMP16 and ITGA4.
    Phosphorylation by PRKCA regulates its subcellular location and function in cell motility.
  • 細胞内局在Apical cell membrane. Cell projection > lamellipodium membrane. Localized at the apical plasma membrane it relocalizes to the lamellipodia of astrocytoma upon phosphorylation by PRKCA. Localizes to the retraction fibers. Localizes to the plasma membrane of oligodendrocytes.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 4732461B14Rik antibody
    • AN2 antibody
    • AN2 proteoglycan antibody
    • Chondroitin sulfate proteoglycan 4 (melanoma-associated) antibody
    • Chondroitin sulfate proteoglycan 4 antibody
    • Chondroitin sulfate proteoglycan NG2 antibody
    • Cspg4 antibody
    • Cspg4 chondroitin sulfate proteoglycan 4 antibody
    • CSPG4_HUMAN antibody
    • HMW-MAA antibody
    • HSN tumor-specific antigen antibody
    • Kiaa4232 antibody
    • MCSP antibody
    • MCSPG antibody
    • MEL-CSPG antibody
    • Melanoma chondroitin sulfate proteoglycan antibody
    • Melanoma-associated chondroitin sulfate proteoglycan antibody
    • MELCSPG antibody
    • MSK16 antibody
    • NG2 antibody
    see all

Anti-NG2 antibody 画像

  • All lanes : Anti-NG2 antibody (ab86067) at 0.04 µg/ml

    Lane 1 : Hela whole cell lysate at 50 µg
    Lane 2 : Hela whole cell lysate at 15 µg
    Lane 3 : Hela whole cell lysate at 5 µg

    Developed using the ECL technique

    Predicted band size : 251 kDa
    Observed band size : 350 kDa (why is the actual band size different from the predicted?)


    Exposure time : 30 seconds
  • Detection of NG2 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab86067 at 10 µg/mg lysate for IP and at 0.4 µg/ml for subsequent Western blot detection.
    Detection: Chemiluminescence with an exposure time of 10 seconds.

Anti-NG2 antibody (ab86067) 使用論文

ab86067 has not yet been referenced specifically in any publications.

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