製品の概要
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製品名Anti-NG2 antibody
NG2 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to NG2
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由来種Rabbit
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アプリケーション適用あり: IHC-FoFr, ICC/IF, WBmore details
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種交差性交差種: Rat, Human
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免疫原
Synthetic peptide conjugated to KLH derived from within residues 1300 - 1400 of Human NG2.
(Peptide available as ab96112.)
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ポジティブ･コントロール
- This antibody gave a positive signal in Human Skin Tissue Lysate - adult normal tissue
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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バッファーPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Immunohistochemistry kits
アプリケーション
Our Abpromise guarantee covers the use of ab83178 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-FoFr | 1/100. | |
| ICC/IF | 1/200. | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 300 kDa (predicted molecular weight: 251 kDa). |
ターゲット情報
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機能Proteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. Binds through its extracellular N-terminus growth factors, extracellular matrix proteases modulating their activity. May regulate MPP16-dependent degradation and invasion of type I collagen participating in melanoma cells invasion properties. May modulate the plasminogen system by enhancing plasminogen activation and inhibiting angiostatin. Functions also as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. May promote retraction fiber formation and cell polarization through Rho GTPase activation. May stimulate alpha-4, beta-1 integrin-mediated adhesion and spreading by recruiting and activating a signaling cascade through CDC42, ACK1 and BCAR1. May activate FAK and ERK1/ERK2 signaling cascades.
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組織特異性Detected only in malignant melanoma cells.
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配列類似性Contains 15 CSPG (NG2) repeats.
Contains 2 laminin G-like domains. -
翻訳後修飾O-glycosylated; contains glycosaminoglycan chondroitin sulfate which are required for proper localization and function in stress fiber formation (By similarity). Involved in interaction with MMP16 and ITGA4.
Phosphorylation by PRKCA regulates its subcellular location and function in cell motility. -
細胞内局在Apical cell membrane. Cell projection > lamellipodium membrane. Localized at the apical plasma membrane it relocalizes to the lamellipodia of astrocytoma upon phosphorylation by PRKCA. Localizes to the retraction fibers. Localizes to the plasma membrane of oligodendrocytes.
- Information by UniProt
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参照データベース
- Entrez Gene: 1464 Human
- Entrez Gene: 81651 Rat
- Omim: 601172 Human
- SwissProt: Q6UVK1 Human
- SwissProt: Q00657 Rat
- Unigene: 513044 Human
- Unigene: 10381 Rat
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別名
- 4732461B14Rik antibody
- AN2 antibody
- AN2 proteoglycan antibody
see all
画像
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Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-NG2 antibody (ab83178)This image is courtesy of an abreview submitted by Ruma Raha-Chowdhury, University Of Cambridge, United KingdomIHC-FoFr image of NG2 staining on sections of rat cortex post injury using ab83178 (1:100). The brain was perfusion fixed using 4% PFA and the sections were permeabilized using 0.1% TritonX in 0.1% PBS. The sections were then blocked using 10% donkey serum for 1hour at 24°C. ab83178 was diluted 1:100 and incubated with the sections for 24 hours at 4°C. The secondary antibody used was donkey polyclonal to rabbit IgG conjugated to Alexa Fluor 488 (1:1000).
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Immunocytochemistry/ Immunofluorescence - Anti-NG2 antibody (ab83178)This image is courtesy of an abreview submitted by Ruma Raha-Chowdhury, University Of Cambridge, United KingdomICC/IF image of ICC/IF staining on miced glia culture cells using ab83178 (1:200). The mixed glia culture was fixed with PFA and the cells were then blocked with 10% donkey serum for 1 hour at 24°C. The cells were then incubated with ab83178 was diluted by 1:200 using 0.3% TritonX with 0.1% PBS and 10% donkey serum. The cells were then incubated with donkey polyclonal to antirabbit conjuaged to Alexa 488 (1:1000). DAPI was used to stain the nucleus. -
Western blot - Anti-NG2 antibody (ab83178)Anti-NG2 antibody (ab83178) at 1 µg/ml + Human skin tissue lysate - total protein (ab30166) at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 251 kDa
Observed band size: 300 kDa (why is the actual band size different from the predicted?)
Additional bands at: 190 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
Human Chondroitin sulfate proteoglycan 4 precursor (NG2) contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
プロトコール
参考文献
This product has been referenced in:
- Ye X et al. Altered ratios of pro- and anti-angiogenic VEGF-A variants and pericyte expression of DLL4 disrupt vascular maturation in infantile haemangioma. J Pathol 239:139-51 (2016). IHC-P ; Human . Read more (PubMed: 26957058) »
- Raha AA et al. The systemic iron-regulatory proteins hepcidin and ferroportin are reduced in the brain in Alzheimer's disease. Acta Neuropathol Commun 1:55 (2013). Read more (PubMed: 24252754) »
レビューと Q&A
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