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Cell extract of HEK293 cells expressing the D3 domain of rat NG2, corresponding to amino acids 1592-2222.
This antibody is derived from the hybridoma 132.38 produced by the fusion of mouse myeloma cells (P3XAg8.653) and splenocytes from BALB/c mice. This antibody is available as an azide-free product (see ab177025).
Our Abpromise guarantee covers the use of ab50009 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 5 µg/ml. Detects a band of approximately 251 kDa (predicted molecular weight: 251 kDa).
Abcam recommends using BSA as the blocking agent.
|IP||Use at an assay dependent concentration.|
|ELISA||Use at an assay dependent concentration.|
|ICC/IF||1/200. PubMed: 18974869|
|IHC-Fr||Use at an assay dependent concentration.|
|IHC-FoFr||Use at an assay dependent concentration.|
ab76897 staining NG2 in rat brain tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.3% Triton and blocked with 5% serum for 1 hour at 24°C. Samples were incubated with primary antibody (1/250) for 16 hours at 4°C. An Alexa Fluor®488 -conjugated Donkey anti-mouse IgG polyclonal (1/500) was used as the secondary antibody.