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Cell extract of HEK293 cells expressing the D3 domain of rat NG2, corresponding to amino acids 1592-2222.
This antibody is derived from the hybridoma 132.38 produced by the fusion of mouse myeloma cells (P3XAg8.653) and splenocytes from BALB/c mice.
The Protocols tab contains a Mouse on Mouse staining protocol with recommendations when using a mouse monoclonal antibody to stain mouse tissues and tips for reducing background.
Our Abpromise guarantee covers the use of ab50009 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 5 µg/ml. Detects a band of approximately 251 kDa (predicted molecular weight: 251 kDa).
Abcam recommends using BSA as the blocking agent.
|IP||Use at an assay dependent concentration.|
|ELISA||Use at an assay dependent concentration.|
|ICC/IF||1/200. PubMed: 18974869|
|IHC-Fr||Use at an assay dependent concentration.|
|IHC-FoFr||Use at an assay dependent concentration.|
ab76897 staining NG2 in rat brain tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.3% Triton and blocked with 5% serum for 1 hour at 24°C. Samples were incubated with primary antibody (1/250) for 16 hours at 4°C. An Alexa Fluor®488 -conjugated Donkey anti-mouse IgG polyclonal (1/500) was used as the secondary antibody.