製品の概要

  • 製品名Anti-NFYC antibody
    NFYC 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to NFYC
  • アプリケーション適用あり: WB, IP, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Ferret, Rhesus monkey, Gorilla
  • 免疫原

    Synthetic peptide corresponding to a region between residue 325 and 375 of Human NFYC (CAI16533.1).

  • ポジティブ・コントロール
    • Whole cell lysate from HeLa cells and 293T cells.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab86237 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 50 kDa).
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能Stimulates the transcription of various genes by recognizing and binding to a CCAAT motif in promoters, for example in type 1 collagen, albumin and beta-actin genes.
  • 配列類似性Belongs to the NFYC/HAP5 subunit family.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CAAT box DNA binding protein subunit C antibody
    • CAAT box DNA-binding protein subunit C antibody
    • CBF C antibody
    • CBFC antibody
    • CCAAT binding factor subunit C antibody
    • CCAAT transcription binding factor subunit gamma antibody
    • DKFZp667G242 antibody
    • FLJ45775 antibody
    • H1TF2A antibody
    • HAP5 antibody
    • histone H1 transcription factor large subunit 2A antibody
    • HSM antibody
    • NF-YC antibody
    • NFYC antibody
    • NFYC_HUMAN antibody
    • Nuclear transcription factor Y gamma antibody
    • Nuclear transcription factor Y subunit C antibody
    • Nuclear transcription factor Y subunit gamma antibody
    • OTTHUMP00000009207 antibody
    • OTTHUMP00000009208 antibody
    • OTTHUMP00000009209 antibody
    • OTTHUMP00000009210 antibody
    • OTTHUMP00000009211 antibody
    • OTTHUMP00000009212 antibody
    • OTTHUMP00000009213 antibody
    • OTTHUMP00000009215 antibody
    • OTTHUMP00000009217 antibody
    • OTTHUMP00000231028 antibody
    • Transactivator HSM 1/2 antibody
    • transactivator HSM-1 antibody
    • Transactivator HSM-1/2 antibody
    see all

Anti-NFYC antibody 画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling NFYC with ab86237 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-NFYC antibody (ab86237) at 0.04 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell lysate from HeLa cells at 5 µg
    Lane 4 : Whole cell lysate from 293T cells at 50 µg

    Developed using the ECL technique

    Predicted band size : 50 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)


    Exposure time : 10 seconds
  • Detection of NFYC in Immunoprecipitates of Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab86237 at 3 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 1 second.

Anti-NFYC antibody (ab86237) 使用論文

ab86237 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab86237.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"