製品の概要

  • 製品名
    Anti-NFYB antibody [NFYBA7]
    NFYB 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [NFYBA7] to NFYB
  • アプリケーション
    適用あり: WB, Dot blotmore details
  • 種交差性
    交差種: Recombinant fragment
    交差が予測される動物種: Human
  • 免疫原

    Recombinant full length protein (Internal sequence).

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at 4°C (stable for up to 12 months). Store at -20°C or -80°C.
  • バッファー
    Preservative: 0.05% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 精製度
    Protein G purified
  • 特記事項(精製)
    Purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG depleted (approximately 95%) fetal bovine serum. Filtered through a 0.22 micrometer membrane.
  • ポリ/モノ
    モノクローナル
  • クローン名
    NFYBA7
  • アイソタイプ
    IgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab51353 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent dilution. Predicted molecular weight: 23 kDa.
Dot blot Use at an assay dependent dilution.

ターゲット情報

  • 機能
    Stimulates the transcription of various genes by recognizing and binding to a CCAAT motif in promoters, for example in type 1 collagen, albumin and beta-actin genes.
  • 配列類似性
    Belongs to the NFYB/HAP3 subunit family.
  • ドメイン
    Can be divided into 3 domains: the weakly conserved A domain, the highly conserved B domain thought to be involved in subunit interaction and DNA binding, and the Glu-rich C domain.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CAAT box DNA binding protein subunit B antibody
    • CAAT box DNA-binding protein subunit B antibody
    • CBF A antibody
    • CBF B antibody
    • CCAAT binding transcription factor subunit A antibody
    • HAP 3 antibody
    • HAP3 antibody
    • NF Y protein chain B antibody
    • NF YB antibody
    • NF-YB antibody
    • NFYB antibody
    • NFYB_HUMAN antibody
    • Nuclear transcription factor Y beta antibody
    • Nuclear transcription factor Y subunit B antibody
    • Nuclear transcription factor Y subunit beta antibody
    • Transcription factor NF Y B subunit antibody
    see all

画像

  • Anti-NFYB antibody [NFYBA7] (ab51353) + immunized recombinant protein

    Predicted band size : 23 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 28 kDa,30 kDa. We are unsure as to the identity of these extra bands.
  • CBB staining of imunized recombinant protein

プロトコール

参考文献

ab51353 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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