製品の概要

  • 製品名Anti-NFKBIL2 antibody
    NFKBIL2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to NFKBIL2
  • アプリケーション適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Horse, Cow, Dog, Leaf frog (Phyllomedusa)
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 500 - 600 of Human NFKBIL2.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in HeLa whole cell lysate and HeLa nuclear lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab101898 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/200. Use paraformaldehyde fixed cells, permeabilized with 0.5% Triton X-100/PBS.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 175 kDa (predicted molecular weight: 151 kDa).

ターゲット情報

  • 機能Component of the MMS22L-TONSL complex, a complex that stimulates the recombination-dependent repair of stalled or collapsed replication forks. The MMS22L-TONSL complex is required to maintain genome integrity during DNA replication by promoting homologous recombination-mediated repair of replication fork-associated double-strand breaks. It may act by mediating the assembly of RAD51 filaments on ssDNA. Within the complex, may act as a scaffold.
  • 組織特異性Expressed in heart, skeletal muscle and tracheal epithelial cells.
  • 配列類似性Belongs to the Tonsoku family.
    Contains 3 ANK repeats.
    Contains 7 LRR (leucine-rich) repeats.
    Contains 8 TPR repeats.
  • ドメインThe ANK repeats mediate the interaction with the MCM complex and histones, while the LRR repeats mediate the interaction with MMS22L.
  • 細胞内局在Cytoplasm. Nucleus. Mainly nuclear. Localizes to DNA damage sites, accumulates at stressed replication forks.
  • Information by UniProt
  • 参照データベース
  • 別名
    • I kappa B related protein antibody
    • I-kappa-B-related protein antibody
    • IkappaBR antibody
    • IKBR antibody
    • Inhibitor of kappa B related protein antibody
    • Inhibitor of kappa B-related protein antibody
    • MMS22L-interacting protein antibody
    • MMS22L-interacting protein, NFBIL2/TONSL antibody
    • NF kappa B inhibitor like protein 2 antibody
    • NF-kappa-B inhibitor-like protein 2 antibody
    • NFBIL2 antibody
    • NFBIL2/TONSL antibody
    • Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor like protein 2 antibody
    • Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor-like 2 antibody
    • Protein IkappaBR antibody
    • Tonsl antibody
    • TONSL_HUMAN antibody
    • Tonsoku-like protein antibody
    • tonsoku-like, DNA repair protein antibody
    see all

Anti-NFKBIL2 antibody 画像

  • All lanes : Anti-NFKBIL2 antibody (ab101898) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 151 kDa
    Observed band size : 175 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 43 kDa,67 kDa,80 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes
  • ab101898 (1/200) staining NFKBIL2 in HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilized with 0.5% Triton X-100/ PBS and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please see Abreview.

    See Abreview

Anti-NFKBIL2 antibody (ab101898) 使用論文

ab101898 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton-X100 in PBS
Username

Dr. Kirk McManus

Verified customer

投稿 Nov 15 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"