製品の概要

  • 製品名Anti-NFKBIL1 antibody
    NFKBIL1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to NFKBIL1
  • アプリケーション適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee, Macaque Monkey, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Human NFKBIL1.

    (Peptide available as ab98906.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following whole cell lysates: HepG2; Jurkat; Raji; Ramos; MOLT4; THP1. This antibody gave a positive result when used in the following methanol fixed cell lines: Malme-3M

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab75491 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
ICC/IF Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能May be a negative regulator of NF-kappa-B activation.
  • 組織特異性Detected in different cell types including monocytes, T-cells, B-cells and hepatocytes.
  • 関連疾患Defects in NFKBIL1 are a cause of susceptibility to rheumatoid arthritis (RA) [MIM:180300]. It is a systemic inflammatory disease with autoimmune features and a complex genetic component. It primarily affects the joints and is characterized by inflammatory changes in the synovial membranes and articular structures, widespread fibrinoid degeneration of the collagen fibers in mesenchymal tissues, and by atrophy and rarefaction of bony structures.
  • 配列類似性Contains 2 ANK repeats.
  • Information by UniProt
  • 参照データベース
  • 別名
    • I-kappa-B-like protein antibody
    • IkappaBL antibody
    • IKBL antibody
    • IKBL1_HUMAN antibody
    • Inhibitor of kappa B-like protein antibody
    • LST1 antibody
    • NF-kappa-B inhibitor-like protein 1 antibody
    • NFKBIL antibody
    • Nfkbil1 antibody
    • Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor-like 1 antibody
    see all

Anti-NFKBIL1 antibody 画像

  • ICC/IF image of ab75491 stained Malme-3M cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab75491 at 5µg/ml overnight at +4°C. The secondary antibody (green) was a goat anti-rabbit DyLight® 488 (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-NFKBIL1 antibody (ab75491) at 1 µg/ml

    Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate
    Lane 4 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
    Lane 5 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate
    Lane 6 : THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 43 kDa
    Observed band size : 43 kDa
    Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 10 minutes

Anti-NFKBIL1 antibody (ab75491) 使用論文

ab75491 has not yet been referenced specifically in any publications.

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