Anti-NFkB p105 / p50 (phospho S337) 抗体 (ab28849)


  • 製品名Anti-NFkB p105 / p50 (phospho S337) antibody
    NFkB p105 / p50 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to NFkB p105 / p50 (phospho S337)
  • アプリケーション適用あり: WB, ELISA, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    The antiserum was produced against synthesized phosphopeptide derived from human NFkappa B p105/p50 around the phosphorylation site of Serine 337.

  • ポジティブ・コントロール
    • WB: MDA cell extract. IHC-P: breast carcinoma.




Our Abpromise guarantee covers the use of ab28849 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/200 - 1/500. Predicted molecular weight: 106 kDa.
ELISA 1/20000.
IHC-P Use at an assay dependent concentration.


  • 機能NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and RelB-p50 complexes are transcriptional activators. The NF-kappa-B p50-p50 homodimer is a transcriptional repressor, but can act as a transcriptional activator when associated with BCL3. NFKB1 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p105 and generation of p50 by a cotranslational processing. The proteasome-mediated process ensures the production of both p50 and p105 and preserves their independent function, although processing of NFKB1/p105 also appears to occur post-translationally. p50 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. In a complex with MAP3K8, NFKB1/p105 represses MAP3K8-induced MAPK signaling; active MAP3K8 is released by proteasome-dependent degradation of NFKB1/p105.
  • 配列類似性Contains 7 ANK repeats.
    Contains 1 death domain.
    Contains 1 RHD (Rel-like) domain.
  • ドメインThe C-terminus of p105 might be involved in cytoplasmic retention, inhibition of DNA-binding, and transcription activation.
    Glycine-rich region (GRR) appears to be a critical element in the generation of p50.
  • 翻訳後修飾While translation occurs, the particular unfolded structure after the GRR repeat promotes the generation of p50 making it an acceptable substrate for the proteasome. This process is known as cotranslational processing. The processed form is active and the unprocessed form acts as an inhibitor (I kappa B-like), being able to form cytosolic complexes with NF-kappa B, trapping it in the cytoplasm. Complete folding of the region downstream of the GRR repeat precludes processing.
    Phosphorylation at 'Ser-903' and 'Ser-907' primes p105 for proteolytic processing in response to TNF-alpha stimulation. Phosphorylation at 'Ser-927' and 'Ser-932' are required for BTRC/BTRCP-mediated proteolysis.
    Polyubiquitination seems to allow p105 processing.
    S-nitrosylation of Cys-61 affects DNA binding.
  • 細胞内局在Nucleus. Cytoplasm. Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DKFZp686C01211 antibody
    • DNA binding factor KBF1 antibody
    • DNA binding factor KBF1 EBP1 antibody
    • DNA-binding factor KBF1 antibody
    • EBP 1 antibody
    • EBP-1 antibody
    • EBP1 antibody
    • KBF1 antibody
    • MGC54151 antibody
    • NF kappa B antibody
    • NF kappaB antibody
    • NF kappabeta antibody
    • NF kB1 antibody
    • NFkappaB antibody
    • NFKB 1 antibody
    • NFKB p105 antibody
    • NFKB p50 antibody
    • Nfkb1 antibody
    • NFKB1_HUMAN antibody
    • Nuclear factor kappa B DNA binding subunit antibody
    • Nuclear factor kappa-B, subunit 1 antibody
    • Nuclear factor NF kappa B p105 subunit antibody
    • Nuclear factor NF kappa B p50 subunit antibody
    • Nuclear factor NF-kappa-B p50 subunit antibody
    • Nuclear factor of kappa light chain gene enhancer in B cells 1 antibody
    • Nuclear factor of kappa light polypeptide gene enhancer in B cells 1 antibody
    • Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 antibody
    • p105 antibody
    • p50 antibody
    • p84/NF-kappa-B1 p98 antibody
    • Transcription factor NFKB1 antibody
    see all

Anti-NFkB p105 / p50 (phospho S337) antibody 画像

  • Ab28849, at a dilution of 1/50, staining p105 in paraffin embedded human breast carcinoma tissue by Immunohistochemistry.
  • All lanes : Anti-NFkB p105 / p50 (phospho S337) antibody (ab28849)

    Lane 1 : Untreated extracts from MDA-MB-435 cells (5-30ug).
    Lane 2 : Extracts from MDA-MB-435 cells (5-30ug) treated with synthesized phosphopeptide.

    Predicted band size : 106 kDa
    Observed band size : 118 kDa (why is the actual band size different from the predicted?)

Anti-NFkB p105 / p50 (phospho S337) antibody (ab28849) 使用論文

This product has been referenced in:
  • Liu Z  et al. Mark4 promotes oxidative stress and inflammation via binding to PPAR? and activating NF-?B pathway in mice adipocytes. Sci Rep 6:21382 (2016). WB ; Mouse . Read more (PubMed: 26888669) »
  • Zheng Y  et al. Therapeutic potential of andrographolide for treating endometriosis. Hum Reprod 27:1300-13 (2012). IHC-P ; Rat . Read more (PubMed: 22402211) »

See all 2 Publications for this product

Product Wall

Thank you for contacting us.

There are 3 antibodies we can offer ab31410, ab28849 and ab7971. The immunogen of these antibodies is significantly similar to Porcine protein so these antibodies might cross r...

Read More