Anti-NFkB p100 / p52 (phospho S872) 抗体 (ab63549)

製品の概要

  • 製品名Anti-NFkB p100 / p52 (phospho S872) antibody
    NFkB p100 / p52 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to NFkB p100 / p52 (phospho S872)
  • 特異性Detects endogenous levels of NFkB p100 only when phosphorylated at serine 872.
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic phosphopeptide derived from human NFkB p100 around the phosphorylation site of serine 872 (S-Q-SP-V-E).

  • ポジティブ・コントロール
    • Extracts from RAW264.7 cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab63549 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 100 kDa (predicted molecular weight: 54 kDa).
ELISA 1/10000.

ターゲット情報

  • 機能NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. In a non-canonical activation pathway, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. The NF-kappa-B heterodimeric RelB-p52 complex is a transcriptional activator. The NF-kappa-B p52-p52 homodimer is a transcriptional repressor. NFKB2 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p100 and generation of p52 by a cotranslational processing. The proteasome-mediated process ensures the production of both p52 and p100 and preserves their independent function. p52 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. p52 and p100 are respectively the minor and major form; the processing of p100 being relatively poor. Isoform p49 is a subunit of the NF-kappa-B protein complex, which stimulates the HIV enhancer in synergy with p65.
  • 関連疾患Note=A chromosomal aberration involving NFKB2 is found in a case of B-cell non Hodgkin lymphoma (B-NHL). Translocation t(10;14)(q24;q32) with IGHA1. The resulting oncogene is also called Lyt-10C alpha variant.
    Note=A chromosomal aberration involving NFKB2 is found in a cutaneous T-cell leukemia (C-TCL) cell line. This rearrangement produces the p80HT gene which encodes for a truncated 80 kDa protein (p80HT).
    Note=In B-cell leukemia (B-CLL) cell line, LB40 and EB308, can be found after heterogeneous chromosomal aberrations, such as internal deletions.
  • 配列類似性Contains 7 ANK repeats.
    Contains 1 death domain.
    Contains 1 RHD (Rel-like) domain.
  • ドメインThe C-terminus of p100 might be involved in cytoplasmic retention, inhibition of DNA-binding by p52 homodimers, and/or transcription activation.
    The glycine-rich region (GRR) appears to be a critical element in the generation of p52.
  • 翻訳後修飾While translation occurs, the particular unfolded structure after the GRR repeat promotes the generation of p52 making it an acceptable substrate for the proteasome. This process is known as cotranslational processing. The processed form is active and the unprocessed form acts as an inhibitor (I kappa B-like), being able to form cytosolic complexes with NF-kappa B, trapping it in the cytoplasm. Complete folding of the region downstream of the GRR repeat precludes processing.
    Subsequent to MAP3K14-dependent serine phosphorylation, p100 polyubiquitination occurs then triggering its proteasome-dependent processing.
    Constitutive processing is tightly suppressed by its C-terminal processing inhibitory domain, named PID, which contains the death domain.
  • 細胞内局在Nucleus. Cytoplasm. Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CVID10 antibody
    • DNA binding factor KBF2 antibody
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    • H2TF1 antibody
    • Lymphocyte translocation chromosome 10 antibody
    • Lymphocyte translocation chromosome 10 protein antibody
    • Lyt 10 antibody
    • Lyt10 antibody
    • NF kB2 antibody
    • NFKB2 antibody
    • NFKB2_HUMAN antibody
    • Nuclear factor NF kappa B p100 subunit antibody
    • Nuclear factor NF kappa B p52 subunit antibody
    • Nuclear factor NF-kappa-B p52 subunit antibody
    • Nuclear factor of kappa light chain gene enhancer in B cells 2 antibody
    • Nuclear factor of kappa light polypeptide gene enhancer in B cells 2 antibody
    • Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 antibody
    • Oncogene Lyt 10 antibody
    • Oncogene Lyt-10 antibody
    • p105 antibody
    • p49/p100 antibody
    see all

Anti-NFkB p100 / p52 (phospho S872) antibody 画像

  • All lanes : Anti-NFkB p100 / p52 (phospho S872) antibody (ab63549) at 1/500 dilution

    Lane 1 : extracts from RAW264.7 cells, treated with EGF (200ng/ml, 30mins)
    Lane 2 : extracts from RAW264.7 cells, treated with EGF (200ng/ml, 30mins) with immunizing peptide


    Predicted band size : 54 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)

Anti-NFkB p100 / p52 (phospho S872) antibody (ab63549) 使用論文

ab63549 has not yet been referenced specifically in any publications.

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