製品の概要

  • 製品名Anti-NFkB Inducing Kinase NIK antibody
    NFkB Inducing Kinase NIK 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to NFkB Inducing Kinase NIK
  • アプリケーション適用あり: ELISA, WB, IHC-Pmore details
  • 種交差性
    交差種: Rat, Human
  • 免疫原

    Synthetic peptide: IFIAGSKQYS QSESLDQIPN NVAHATEGKM ARVCWKGKRR SKARKKRKKK SSKSLAHAGV ALAKPLPRTP EQESCTIPVQ EDESPLGAPY VRNTPQFTKP L conjugated to KLH, corresponding to amino acids 100-200 of Human NFkB Inducing Kinase NIK

  • ポジティブ・コントロール
    • 293 cell lysate. Rat testis tissue lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab37712 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/1000.
WB 1/100 - 1/500. Predicted molecular weight: 104 kDa.
IHC-P 1/50 - 1/100.

ターゲット情報

  • 機能Lymphotoxin beta-activated kinase which seems to be exclusively involved in the activation of NF-kappa-B and its transcriptional activity. Promotes proteolytic processing of NFKB2/P100, which leads to activation of NF-kappa-B via the non-canonical pathway. Could act in a receptor-selective manner.
  • 組織特異性Weakly expressed in testis, small intestine, spleen, thymus, peripheral blood leukocytes, prostate, ovary and colon.
  • 配列類似性Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
    Contains 1 protein kinase domain.
  • 翻訳後修飾Autophosphorylated. Phosphorylation at Thr-559 is required to activates its kinase activity and 'Lys-63'-linked polyubiquitination. Phosphorylated by CHUK/IKKA leading to MAP3K14 destabilization.
    Ubiquitinated. Undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination. 'Lys-48'-linked polyubiquitination leads to its degradation by the proteasome, while 'Lys-63'-linked polyubiquitination stabilizes and activates it.
  • 細胞内局在Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • MAP3K14 antibody
    • FTDCR1B antibody
    • HS antibody
    • HsNIK antibody
    • M3K14_HUMAN antibody
    • Map3k14 antibody
    • Mitogen activated protein kinase kinase kinase 14 antibody
    • Mitogen-activated protein kinase kinase kinase 14 antibody
    • NF kappa beta inducing kinase antibody
    • NF-kappa-beta-inducing kinase antibody
    • NIK antibody
    • Serine/threonine-protein kinase NIK antibody
    see all

Anti-NFkB Inducing Kinase NIK antibody 画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labeling NFkB Inducing Kinase NIK with ab37712 at 1/25. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at 37°C. Heat mediated antigen retrieval was performed using a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A Peroxidase-conjugated Goat anti-rabbit polyclonal (ready to use) was used as the secondary antibody.

  • All lanes : Anti-NFkB Inducing Kinase NIK antibody (ab37712) at 1/1000 dilution

    Lane 1 : 293 whole cell lysate
    Lane 2 : rat testis tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

    Predicted band size : 104 kDa
    Observed band size : 104 kDa
    Additional bands at : 37 kDa,60 kDa. We are unsure as to the identity of these extra bands.

    Blocking/Dilution buffer: 5% NFDM/TBST.

Anti-NFkB Inducing Kinase NIK antibody (ab37712) 使用論文

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Thank you very much for your call earlier this week and for your patience while I've been in touch with my colleagues regarding your enquiry.

I've confirmed that ab37712 was tested with non-transfected 293 lysate, however ab19144 was actually...

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