Anti-NFkB Inducing Kinase NIK 抗体 (ab19144)


  • 製品名Anti-NFkB Inducing Kinase NIK antibody
    NFkB Inducing Kinase NIK 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to NFkB Inducing Kinase NIK
  • 特異性This antibody recognises human NFkB Inducing Kinase NIK.
  • アプリケーション適用あり: IHC-P, ICC/IF, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide:


    , corresponding to amino acids 931-947 of Human NFkB Inducing Kinase NIK

  • ポジティブ・コントロール
    • Whole cell lysate of 293.




Our Abpromise guarantee covers the use of ab19144 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 5 µg/ml.
WB 1/500. Detects a band of approximately 105 kDa (predicted molecular weight: 105.4 kDa).Can be blocked with NFkB Inducing Kinase NIK peptide (ab8385).


  • 機能Lymphotoxin beta-activated kinase which seems to be exclusively involved in the activation of NF-kappa-B and its transcriptional activity. Promotes proteolytic processing of NFKB2/P100, which leads to activation of NF-kappa-B via the non-canonical pathway. Could act in a receptor-selective manner.
  • 組織特異性Weakly expressed in testis, small intestine, spleen, thymus, peripheral blood leukocytes, prostate, ovary and colon.
  • 配列類似性Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
    Contains 1 protein kinase domain.
  • 翻訳後修飾Autophosphorylated. Phosphorylation at Thr-559 is required to activates its kinase activity and 'Lys-63'-linked polyubiquitination. Phosphorylated by CHUK/IKKA leading to MAP3K14 destabilization.
    Ubiquitinated. Undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination. 'Lys-48'-linked polyubiquitination leads to its degradation by the proteasome, while 'Lys-63'-linked polyubiquitination stabilizes and activates it.
  • 細胞内局在Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • MAP3K14 antibody
    • FTDCR1B antibody
    • HS antibody
    • HsNIK antibody
    • M3K14_HUMAN antibody
    • Map3k14 antibody
    • Mitogen activated protein kinase kinase kinase 14 antibody
    • Mitogen-activated protein kinase kinase kinase 14 antibody
    • NF kappa beta inducing kinase antibody
    • NF-kappa-beta-inducing kinase antibody
    • NIK antibody
    • Serine/threonine-protein kinase NIK antibody
    see all

Anti-NFkB Inducing Kinase NIK antibody 画像

  • Anti-NFkB Inducing Kinase NIK antibody (ab19144) at 1/500 dilution + NIK-transfected 293 cell lysate

    Predicted band size : 105.4 kDa
  • ICC/IF image of ab19144 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab19144, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC image of ab19144 staining in human cervix formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab19144, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-NFkB Inducing Kinase NIK antibody (ab19144) 使用論文

ab19144 has not yet been referenced specifically in any publications.

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Thank you very much for your call earlier this week and for your patience while I've been in touch with my colleagues regarding your enquiry.

I've confirmed that ab37712 was tested with non-transfected 293 lysate, however ab19144 was actually...

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I have received feedback from the laboratory who understand why you are concerned about your results as the positive control normally gives a very nice strong band at 105kDa. I have added the image of the lab on the datasheet to show you our results. W...

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