Anti-NFkB Inducing Kinase NIK 抗体 (ab19144)
Key features and details
- Rabbit polyclonal to NFkB Inducing Kinase NIK
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-NFkB Inducing Kinase NIK antibody
NFkB Inducing Kinase NIK 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to NFkB Inducing Kinase NIK -
由来種
Rabbit -
特異性
This antibody recognises human NFkB Inducing Kinase NIK. -
アプリケーション
適用あり: IHC-P, ICC/IF, WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide:
SFAWSWRVKHGQLENRP
, corresponding to amino acids 931-947 of Human NFkB Inducing Kinase NIK -
ポジティブ・コントロール
- Whole cell lysate of 293.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab19144の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
Use a concentration of 1 - 5 µg/ml.
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WB |
1/500. Detects a band of approximately 105 kDa (predicted molecular weight: 105.4 kDa).
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特記事項 |
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IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
Use a concentration of 1 - 5 µg/ml. |
WB
1/500. Detects a band of approximately 105 kDa (predicted molecular weight: 105.4 kDa). |
ターゲット情報
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機能
Lymphotoxin beta-activated kinase which seems to be exclusively involved in the activation of NF-kappa-B and its transcriptional activity. Promotes proteolytic processing of NFKB2/P100, which leads to activation of NF-kappa-B via the non-canonical pathway. Could act in a receptor-selective manner. -
組織特異性
Weakly expressed in testis, small intestine, spleen, thymus, peripheral blood leukocytes, prostate, ovary and colon. -
配列類似性
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
Contains 1 protein kinase domain. -
翻訳後修飾
Autophosphorylated. Phosphorylation at Thr-559 is required to activates its kinase activity and 'Lys-63'-linked polyubiquitination. Phosphorylated by CHUK/IKKA leading to MAP3K14 destabilization.
Ubiquitinated. Undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination. 'Lys-48'-linked polyubiquitination leads to its degradation by the proteasome, while 'Lys-63'-linked polyubiquitination stabilizes and activates it. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 9020 Human
- Omim: 604655 Human
- SwissProt: Q99558 Human
- Unigene: 404183 Human
- Unigene: 668927 Human
- Unigene: 735695 Human
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別名
- MAP3K14 antibody
- FTDCR1B antibody
- HS antibody
see all
画像
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Anti-NFkB Inducing Kinase NIK antibody (ab19144) at 1/500 dilution + NIK-transfected 293 cell lysate
Predicted band size: 105.4 kDa -
ICC/IF image of ab19144 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab19144, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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IHC image of ab19144 staining in human cervix formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab19144, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (4)
ab19144 は 4 報の論文で使用されています。
- Li J et al. Hypoxic colorectal cancer-derived extracellular vesicles deliver microRNA-361-3p to facilitate cell proliferation by targeting TRAF3 via the noncanonical NF-?B pathways. Clin Transl Med 11:e349 (2021). PubMed: 33784010
- Das R et al. Lymphotoxin-ß receptor-NIK signaling induces alternative RELB/NF-?B2 activation to promote metastatic gene expression and cell migration in head and neck cancer. Mol Carcinog 58:411-425 (2019). PubMed: 30488488
- Maracle CX et al. Noncanonical NF-?B signaling in microvessels of atherosclerotic lesions is associated with inflammation, atheromatous plaque morphology and myocardial infarction. Atherosclerosis 270:33-41 (2018). IHC-P ; Human . PubMed: 29407886
- Maracle CX et al. Silencing NIK potentiates anti-VEGF therapy in a novel 3D model of colorectal cancer angiogenesis. Oncotarget 9:28445-28455 (2018). PubMed: 29983872